Effective Separation of Cancer‐Derived Exosomes in Biological Samples for Liquid Biopsy: Classic Strategies and Innovative Development. Issue 9 (9th May 2022)

• Record Type: Journal Article
• Authors: Xie, Yujiao; Xu, Xiawei; Lin, Jie; Xu, Yanping; Wang, Jing; Ren, Yong; Wu, Aiguo
• Is Part Of: Global challenges. Volume 6:Issue 9(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-05-09
• Subjects: cancers -- cancer‐derived exosomes -- liquid biopsies -- separation methods; Climatic changes -- Periodicals; Sustainable development -- Periodicals; Globalization -- Environmental aspects -- Periodicals; Electronic journals; Periodicals; 500
• DOI: 10.1002/gch2.202100131
• Languages: English
• ISSNs: 2056-6646

Abstract

Abstract: Liquid biopsy has remarkably facilitated clinical diagnosis and surveillance of cancer via employing a non‐invasive way to detect cancer‐derived components, such as circulating tumor DNA and circulating tumor cells from biological fluid samples. The cancer‐derived exosomes, which are nano‐sized vesicles secreted by cancer cells have been investigated in liquid biopsy as their important roles in intracellular communication and disease development have been revealed. Given the challenges posed by the complicated humoral microenvironment, which contains a variety of different cells and macromolecular substances in addition to the exosomes, it has attracted a large amount of attention to effectively isolate exosomes from collected samples. In this review, the authors aim to analyze classic strategies for separation of cancer‐derived exosomes, giving an extensive discussion of advantages and limitations of these methods. Furthermore, the innovative multi‐strategy methods to realize efficient isolation of cancer‐derived exosomes in practical applications are also presented. Additionally, the possible development trends of exosome separation in to the future is discussed in this review. Abstract : This paper provides an overview of the latest advances in separation of cancer‐derived exosomes with a discussion of the advantages and limitations of each method. The work highlights the importance of combining microfluidics with multiple separation strategies to develop integrated microsystems for rapid separation of exosomes with high stability and sensitivity, which will ultimately enable a facile and effective operation for early diagnosis and cancer treatment.