DAMPS and complement activation in platelet concentrates that induce adverse reactions in patients. Issue 9 (11th August 2022)

• Record Type: Journal Article
• Authors: de Wit, Yasmin E. S.; Hamzeh‐Cognasse, Hind; Cognasse, Fabrice; ten Brinke, Anja; Zeerleder, Sacha S.
• Is Part Of: Transfusion. Volume 62:Issue 9(2022)
• Page Start: 1721
• Page End: 1726
• Publication Date: 2022-08-11
• Subjects: complement activation -- DAMPS -- febrile non‐hemolytic reaction -- HMGB1 -- platelet concentrates -- transfusion -- transfusion adverse reactions; Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.17061
• Languages: English
• ISSNs: 0041-1132

Abstract

Abstract: Background: Patients with severe thrombocytopenia due to bone marrow failure and after chemotherapy are still treated with platelet transfusions. Platelet concentrates (PC) are associated with a high incidence of adverse reactions (AR). Platelet‐derived damage‐associated molecular patterns (DAMPS) and complement were proposed to play a role in the pathology of AR. Study Design and Methods: Single donor apheresis platelet concentrates (SDA PCs) were produced in a regional setting of the French Blood Establishment. After transfusion samples were collected from PC and possible AR in patients were recorded. Platelet activation markers, High mobility group box 1 (HMGB1) and complement activation products (CAP) were measured. The correlation between platelet activation, and HMGB1 and complement activation was analyzed. Results: A total of 56 PC were included in the study. 30 PC induced no AR, and 26 induced AR (Febrile non‐hemolytic transfusion reaction n = 16; Atypical Allergic Transfusion Reactions n = 11; hemodynamic instability n = 5) in the patients. The levels of P‐selectin, sCD40L, HMGB1, C3b/c, and C4b/c were all significantly increased in PC that induced AR following transfusion in patients. Additionally, HMGB1, C3b/c, and C4b/c were positively correlated with P‐selectin and sCD40L. Conclusion: In this study, we observed an association between HMGB1 and CAP and the incidence of AR. Furthermore, we demonstrated that both HMGB1 and complement activation were correlated to platelet activation.