Transcriptionally amplified synthesis of fluorogenic RNA aptamers for label-free DNA glycosylase assay. Issue 73 (25th August 2022)

• Record Type: Journal Article
• Authors: Ma, Fei; Liu, Ya-Zhen; Liu, Meng; Qiu, Jian-Ge; Zhang, Chun-Yang
• Is Part Of: Chemical communications. Volume 58:Issue 73(2022)
• Page Start: 10229
• Page End: 10232
• Publication Date: 2022-08-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d2cc03628b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We demonstrate the base excision-controlled amplified synthesis of fluorogenic RNA for label-free biosensing of uracil-DNA glycosylase. Abstract : We demonstrate for the first time the utilization of fluorogenic RNA aptamers for label-free uracil-DNA glycosylase (UDG) assay. Through rationally engineering the transcription machine with dU substitution, this assay requires only a single probe to simultaneously sense and amplify the UDG signal, achieving a low detection limit of 6.3 × 10 −6 U mL −1 . Moreover, it can be applied for screening UDG inhibitors and measuring endogenous UDG activity in different cells.