Zinc oxide nanoparticles (ZnO-NPs) exhibit immune toxicity to crucian carp (Carassius carassius) by neutrophil extracellular traps (NETs) release and oxidative stress. Issue 129 (October 2022)

• Record Type: Journal Article
• Authors: Hong, Hongrong; Liu, Ziyi; Li, Shuangqiu; Wu, Di; Jiang, Liqiang; Li, Peixuan; Wu, Zhikai; Xu, Jingnan; Jiang, Aimin; Zhang, Yong; Wei, Zhengkai; Yang, Zhengtao
• Is Part Of: Fish & shellfish immunology. Issue 129(2022)
• Page Start: 22
• Page End: 29
• Publication Date: 2022-10
• Subjects: Zinc oxide nanoparticles -- Deoxyribonuclease I -- Immunotoxicity -- Glycolysis -- Liver injury; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2022.07.025
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Zinc oxide nanoparticles (ZnO-NPs) are widely used in sunscreens, cosmetics, paint, construction materials, and other products. ZnO-NPs released into the environment can harm aquatic creatures and pose a health risk to humans through the food chain. ZnO-NPs are toxic to fish, but there are few reports on its immunotoxicity on crucian carp ( Carassius carassius ). In this study, ZnO-NPs increased the biochemical indexes of the liver in serum, including aspartate aminotransferase (AST) and alanine aminotransferase (ALT). In histopathological observation, many inflammatory cells were filled in the liver's central vein stimulated by ZnO-NPs. Furthermore, ZnO-NPs could increase malondialdehyde (MDA) level, lessen superoxide dismutase (SOD) level, and elevate the level of neutrophil extracellular traps (NETs). However, deoxyribonuclease I (DNase I) alleviated all biochemical indexes and histopathological changes. Immunofluorescence in vitro confirmed that NETs were composed of citrullinated histone 3, myeloperoxidase, and neutrophil elastase. ZnO-NPs-increased NETs were dependent on reactive oxygen species (ROS) and nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase and were also related to partial processes of glycolysis. Our study confirms that ZnO-NPS has a toxic effect on the liver of crucian carp. DNase I can prevent liver damage caused by ZnO-NPs, which provides a new insight into the immunotoxicity of ZnO-NPs to fish. Graphical abstract: Image 1 Highlights: Vivo experiment confirmed that ZnO-NPs caused Organ injury in crucian carp related to NETs. Vitro experiment validated that ZnO-NPs induce NETs production in crucian carp neutrophils. ZnO-NPs developed NETs in crucian carp to form ERK1/2 and p38 dependent signaling pathways. The form of NETs in crucian carp induced by ZnO-NPs depended on NADPH oxidase and glycolysis. DNase I is Promising for prevention of liver injury induced by ZnO-NPs.