Funneling lignin-derived compounds into polyhydroxyalkanoate by Halomonas sp. Y3. (October 2022)

• Record Type: Journal Article
• Authors: Tang, Hao; Wang, Ming-Jun; Gan, Xiao-Feng; Li, Yuan-Qiu
• Is Part Of: Bioresource technology. Volume 362(2022)
• Publication Date: 2022-10
• Subjects: Lignin-derived compounds -- Conversion -- Polyhydroxyalkanoate -- Halomonas sp. Y3 -- Genome; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127837
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Halomonas sp. Y3 degraded lignin and produced PHA from lignin. Halomonas sp. Y3 efficiently converted lignin-derived compounds into PHA. Genome of Halomonas sp. Y3 revealed a biological funneling pathway for LDCs into PHA. An open unsterile and efficient PHA production was achieved. Abstract: Lignin-derived compounds (LDCs) biological funneling for polyhydroxyalkanoate (PHA) synthesis has been attractive but elusive. Herein, the Halomonas sp. Y3 is isolated and developed for PHA production from LDCs. Of the tested 13 LDCs, 4-hydroxybenzoic acid (4-HBA), protocatechuate (PA), catechol (CAT), and vanillic acid (VA) exhibit a hyper-degradation and production with 87.2 %, 85.8 %, 84.7 %, and 83.4 % TOC removal rate and 535.2 mg/L, 506.5 mg/L, 435.6 mg/L, and 440.8 mg/L PHA concentration, respectively. The Halomonas sp. Y3 genome is sequenced by identifying numerous genes responsible for LDCs funneling, stress response, and PHA biosynthesis. An open unsterilized fermentation with optimal conditions of pH 9.0 and NaCl 60 g/L is investigated, achieving a completely aseptic effect and significantly improved PHA production from LDCs. Overall, the results indicate that the Halomonas sp. Y3 is an ideal candidate for LDC bioconversion and exhibits a great potential to realize black liquor valorization.