Direct conversion of carbon dioxide to glucose using metabolically engineered Cupriavidus necator. (October 2022)

• Record Type: Journal Article
• Authors: Wang, Xiaolu; Luo, Huiying; Wang, Yaru; Wang, Yuan; Tu, Tao; Qin, Xing; Su, Xiaoyun; Huang, Huoqing; Bai, Yingguo; Yao, Bin; Zhang, Jie
• Is Part Of: Bioresource technology. Volume 362(2022)
• Publication Date: 2022-10
• Subjects: Cupriavidus necator -- Metabolic engineering -- CO2 -- Glucose -- HAD phosphatase; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.127806
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The metabolically engineered C. necator can convert CO2 to glucose directly. The conversion process is independent of chemical or electrochemical CO2 fixation. Deletion of glk gene generated a glucose-producing strain. The maximum glucose titer from CO2 reached 253.3 mg/L. Abstract: Artificial synthesis of glucose, the monomer of starch, from renewable resources and CO2 is a promising method for addressing food crisis and alleviating climate change. Here, the construction of a microbial biocatalyst for glucose production from renewable resources and CO2 was reported. Initially, blocking the glucose catabolic pathway via deletion of glk gene generated a glucose-producing strain of Cupriavidus necator with titers of 24.7, 47.5 and 180.1 mg/L from fructose, glycerol and CO2, respectively. Subsequently, the Entner-Doudoroff pathway and polyhydroxybutyrate biosynthesis pathway were disrupted to further increase glucose accumulation. The maximum glucose titer and yield on biomass from CO2 reached 253.3 mg/L and 91.6 mg/L/OD600, respectively. Finally, the phosphatases that mediate the dephosphorylation of phosphorylated glucose were identified. Overexpression of HAD1 and cbbY2 could enhance glucose titer by 5.5-fold when fructose was used as sole carbon source. This study demonstrates a feasible route for microbial-based synthesis of glucose from CO2 .