Seahorse brood pouch morphology and control of male parturition in Hippocampus abdominalis. (September 2022)

• Record Type: Journal Article
• Authors: Dudley, J.S.; Paul, J.W.; Teh, V.; Mackenzie, T.E.; Butler, T.A.; Tolosa, J.M.; Smith, R.; Foley, M.; Dowland, S.; Thompson, M.B.; Whittington, C.M.
• Is Part Of: Placenta. Volume 127(2022)
• Page Start: 88
• Page End: 94
• Publication Date: 2022-09
• Subjects: Anal fin musculature -- Brood pouch -- Contraction assay -- Labour -- μ-CT -- Syngnathid -- Pregnancy; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2022.07.015
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: Syngnathids (seahorses, pipefishes and seadragons) are among the few vertebrates that display male pregnancy. During seahorse pregnancy, males incubate developing embryos embedded in a placenta within a fleshy brood pouch, before expelling fully developed neonates at parturition. The mechanisms underpinning seahorse parturition are poorly understood. Methods: We examined the morphology of the brood pouch using microcomputed tomography and histological techniques, in combination with physiological assays, to examine how male pot-bellied seahorses ( Hippocampus abdominalis ) control labour. In female-pregnant vertebrates, nonapeptide hormones (such as vasopressin- and oxytocin-like hormones) produce contractions of gestational smooth muscle to produce labour. Results: Histological analysis of the seahorse brood pouch reveals only scattered small smooth muscle bundles in the brood pouch, and in-vitro application of isotocin (a teleost nonapeptide hormone) to the brood pouch do not produce measurable muscle contractions. Micro-computed tomography shows differences in size and orientation of the anal fin assembly between male and female pot-bellied seahorses, and histological analysis reveals large skeletal muscle bundles attached to the anal fin bones at the male brood pouch opening. Discussion: We conclude that seahorse parturition may be facilitated by contraction of these muscles, which, in combination with body movements, serves to gape open the pouch and expel the neonates. Future biomechanical studies are needed to test this hypothesis. Highlights: Male seahorses incubate embryos inside a brood pouch via a placenta. We examined the mechanisms by which parturition is controlled in male seahorses. In vitro application of isotocin did not produce measurable brood pouch contractions. A modified anal fin assembly in males may act in combination with body movements to expel neonates. Provides evidence for divergent mechanisms underpinning parturition in pregnant vertebrates.