Inorganic quantum dots - anammox consortia hybrid for stable nitrogen elimination under high-intensity solar-simulated irradiation. (1st September 2022)

• Record Type: Journal Article
• Authors: Ren, Zhi-Qi; Yu, Lin-Qian; Wang, Hao; Li, Gui-Feng; Zhang, Li-Ge; Du, Xue-Ning; Huang, Bao-Cheng; Jin, Ren-Cun
• Is Part Of: Water research. Volume 223(2022)
• Publication Date: 2022-09-01
• Subjects: Anammox -- Photoinhibition -- Se-CQDs -- ROS -- Oxidative stress -- Antioxidant enzymes; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2022.119033
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: High-intense light would inhibit anammox sludge activity decline. Light induced · OH and · O2 − were able to destruct ladderane lipids structure. Se-CQDs would greatly mitigate the photoinhibition and maintain a stable performance. The potential mitigation mechanism by Se-CQDs was clarified. Abstract: External stimulus such as light irradiation is able to deteriorate intracellular redox homeostasis and induce photooxidative damage to non-photogenic bacteria. Exploiting effective strategies to help bacteria resisting infaust stress is meaningful for achieving a stable operation of biological treatment system. In this work, selenium-doped carbon quantum dots (Se-CQDs) were blended into anaerobic ammonia oxidation (anammox) bacteria and an inorganic nanoparticle-microbe hybrid was successfully fabricated to evaluate its nitrogen removal performance under solar-simulated irradiation. It was found that the specific anammox activity decreased by 29.7 ± 5.2% and reactive oxygen species (ROS) content increased by 134.8 ± 4.1% under 50, 000 lux light. Sludge activity could be completely recovered under the optimum dosage of 0.42 mL·(g volatile suspended solid) -1 Se-CQDs. Hydroxyl radical (·OH) and superoxide anion radical (·O2 − ) were identified as the leading ROS inducing lipid peroxidation and antioxidase function detriment. Also, the structure of ladderane lipids located on anammoxosome was destroyed by ROS and functional genes abundances declined accordingly. Although cell surface coated Se-CQDs could absorb ultraviolet light and partially mitigated the photoinhibition, the direct scavenging of ROS by intracellular Se-CQDs primarily contributed to the cellular redox homeostasis, antioxidase activity recovery and sludge activity improvement. The findings of this work provide in-depth understanding the metabolic response mechanism of anammox consortia to light irradiation and might be valuable for a more stable and sustainable nitrogen removal technology, i.e., algal-bacterial symbiotic system, development. Graphical abstract: Image, graphical abstract