Identification of the cuticle protein AccCPR2 gene in Apis cerana cerana and its response to environmental stress. Issue 5 (20th June 2022)

• Record Type: Journal Article
• Authors: Tan, Shuai; Li, Guilin; Guo, Hengjun; Li, Han; Tian, Ming; Liu, Qingxin; Wang, Ying; Xu, Baohua; Guo, Xingqi
• Is Part Of: Insect molecular biology. Volume 31:Issue 5(2022)
• Page Start: 634
• Page End: 646
• Publication Date: 2022-06-20
• Subjects: antioxidant -- Apis cerana cerana -- cuticular proteins -- environmental stresses -- expression analysis -- RNA interference; Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12792
• Languages: English
• ISSNs: 0962-1075

Abstract

Abstract: Cuticular proteins (CPs) are known to play important roles in insect development and defence responses. The loss of CP genes can lead to changes in insect morphology and sensitivity to the external environment. In this study, we identified the AccCPR2 gene, which belongs to the CPR family (including the R&R consensus motif) of CPs, and explored its function in the response of Apis cerana cerana to adverse external stresses. Our results demonstrated that AccCPR2 was highly expressed in the late pupal stage and epidermis, and the expression of AccCPR2 may be induced or inhibited under different stressors. RNA interference experiments showed that knockdown of AccCPR2 reduced the activity of antioxidant enzymes, led to the accumulation of oxidative damage and suppressed the expression of several antioxidant genes. In addition, knockdown of AccCPR2 also reduced the pesticide resistance of A. cerana cerana . The overexpression of AccCPR2 in a prokaryotic system further confirmed its role in resistance to various stresses. In summary, AccCPR2 may play pivotal roles in the normal development and environmental stress response of A. cerana cerana . This study also enriched the theoretical knowledge of the resistance biology of bees. Abstract : Survival curves of Apis cerana cerana fed glyphosate (a) and glyphosate isopropylamine (b) after AccCPR2 silencing. CK represents the group that was not fed double‐stranded RNA. The survival curve was generated using GraphPad Prism (version 8.0.2) analysis software.