Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis. Issue 9 (24th May 2021)

• Record Type: Journal Article
• Authors: Antoniadi, Ioanna; Skalický, Vladimír; Sun, Guiling; Ma, Wen; Galbraith, David W.; Novák, Ondřej; Ljung, Karin
• Other Names: Galbraith David guestEditor.; Loureiro João guestEditor.
• Is Part Of: Cytometry. Volume 101:Issue 9(2022)
• Page Start: 725
• Page End: 736
• Publication Date: 2021-05-24
• Subjects: autofluorescence -- best practices -- plant flow cytometry and sorting -- protoplasts -- viability and integrity; Flow cytometry -- Periodicals; Imaging systems in biology -- Periodicals; Imaging systems in medicine -- Periodicals; Diagnostic imaging -- Periodicals; 571.605
• DOI: 10.1002/cyto.a.24461
• Languages: English
• ISSNs: 1552-4922

Abstract

Abstract: Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single‐cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single‐cell suspensions can be obtained through digestion of the cells walls and release of the so‐called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.