Native glycosylation and binding of the antidepressant paroxetine in a low‐resolution crystal structure of human myeloperoxidase. Issue 9 (9th August 2022)

• Record Type: Journal Article
• Authors: Krawczyk, Lucas; Semwal, Shubham; Soubhye, Jalal; Lemri Ouadriri, Salma; Prévost, Martin; Van Antwerpen, Pierre; Roos, Goedele; Bouckaert, Julie
• Is Part Of: Acta crystallographica. Volume 78:Issue 9(2022)
• Page Start: 1099
• Page End: 1109
• Publication Date: 2022-08-09
• Subjects: human myeloperoxidase -- glycosylation -- N‐glycan refinement -- thiocyanate -- paroxetine; X-ray crystallography -- Periodicals; Crystallography -- Periodicals; Molecular biology -- Periodicals; Molecular structure -- Periodicals; Biomolecules -- Structure -- Periodicals; Cytology -- Periodicals; Biomolecules -- Structure; Crystallography; Cytology; Molecular biology; Molecular structure; X-ray crystallography; Periodicals; 548
• DOI: 10.1107/S2059798322007082
• Languages: English
• ISSNs: 2059-7983

Abstract

Abstract : Myeloperoxidase, prepared from human neutrophil granulocytes, was crystallized in complex with the serotonin‐transporter inhibitor paroxetine in crystals containing eight monomers in the asymmetric unit. Each protomer shows up to five asparagine‐linked glycan structures. The strategies used and the difficulties encountered in the building and refinement of glycosylation for their improved presentation in the PDB are explained. Abstract : Human myeloperoxidase (MPO) utilizes hydrogen peroxide to oxidize organic compounds and as such plays an essential role in cell‐component synthesis, in metabolic and elimination pathways, and in the front‐line defence against pathogens. Moreover, MPO is increasingly being reported to play a role in inflammation. The enzymatic activity of MPO has also been shown to depend on its glycosylation. Mammalian MPO crystal structures deposited in the Protein Data Bank (PDB) present only a partial identification of their glycosylation. Here, a newly obtained crystal structure of MPO containing four disulfide‐linked dimers and showing an elaborate collection of glycans is reported. These are compared with the glycans identified in proteomics studies and from 18 human MPO structures available in the PDB. The crystal structure also contains bound paroxetine, a blocker of serotonin reuptake that has previously been identified as an irreversible inhibitor of MPO, in the presence of thiocyanate, a physiological substrate of MPO.