Flow cytometric resolution of yeast is affected by enzymatic treatment and culture media. Issue 9 (2nd August 2022)

• Record Type: Journal Article
• Authors: Wang, Mei; Wu, Feizhen; Gu, Zhongkai
• Other Names: Wlodawer Alexander guestEditor.
• Is Part Of: FEBS open bio. Volume 12:Issue 9(2022)
• Page Start: 1623
• Page End: 1633
• Publication Date: 2022-08-02
• Subjects: cell cycle -- cytometric resolution -- flow cytometry -- Saccharomyces cerevisiae -- SYBR Green I -- yeast; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13456
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Saccharomyces cerevisiae is an important model organism and a typical fungal representative for studies of eukaryotes. The cell cycle of yeast can be analyzed by flow cytometry, and refining the cytometric resolution of the cell cycle with this technique is important. Such refinement is potentially influenced by multiple factors, including enzymatic treatment and variations in the culture media used, although this has been subject to only limited investigation. Here, we examined the effect of different enzymatic pre‐treatments and various media on cytometric resolution. We show that cytometric resolution is significantly altered by both enzymatic conditions and the media used. Culture media with different amino nitrogen concentrations potentially impact the protein content in the yeast cell wall, which may affect the permeability of the cell wall and alter cytometric resolution. The present study provides beneficial technical information about the influence of media and enzymes on the cytometric resolution of the yeast cell cycle and most likely other fungi, which should be considered in future research. Abstract : The cytometric resolution of the cell cycle of yeast as measured by flow cytometry is influenced by both enzymatic conditions and the culture medium used. This phenomenon may be a result of variations in the protein ratio in the cell wall, and this fluctuation in cytometric resolution should be considered for future studies.