A Sandwich‐Type Electrochemical Immunosensor for Insulin Detection Based on Au‐Adhered Cu5Zn8 Hollow Porous Carbon Nanocubes and AuNP Deposited Nitrogen‐Doped Holey Graphene. Issue 35 (11th August 2022)

• Record Type: Journal Article
• Authors: Sakthivel, Rajalakshmi; Prasanna, Sanjay Ballur; Tseng, Ching‐Li; Lin, Lu‐Yin; Duann, Yeh‐Fang; He, Jr‐Hau; Chung, Ren‐Jei
• Is Part Of: Small. Volume 18:Issue 35(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-08-11
• Subjects: Cu 5Zn 8 hollow porous carbon nanocube -- insulin -- nitrogen‐doped holey graphene -- sandwich‐type electrochemical immunosensor -- sensing platform; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.202202516
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: Rapid, accurate, and sensitive insulin detection is crucial for managing and treating diabetes. A simple sandwich‐type electrochemical immunosensor is engineered using gold nanoparticle (AuNP)‐adhered metal–organic framework‐derived copper–zinc hollow porous carbon nanocubes (Au@Cu5 Zn8 /HPCNC) and AuNP‐deposited nitrogen‐doped holey graphene (NHG) are used as a dual functional label and sensing platform. The results show that identical morphology and size of Au@Cu5 Zn8 /HPCNC enhance the electrocatalytic active sites, conductivity, and surface area to immobilize the detection antibodies (Ab2 ). In addition, AuNP/NHG has the requisite biocompatibility and electrical conductivity, which facilitates electron transport and increases the surface area of the capture antibody (Ab1 ). Significantly, Cu5 Zn8 /HPCNC exhibits necessary catalytic activity and sensitivity for the electrochemical reduction of H2 O2 using ( i – t ) amperometry and improves the electrochemical response in differential pulse voltammetry. Under optimal conditions, the immunosensor for insulin demonstrates a wide linear range with a low detection limit and viable specificity, stability, and reproducibility. The platform's practicality is evaluated by detecting insulin in human serum samples. All these characteristics indicate that the Cu5 Zn8 /HPCNC‐based biosensing strategy may be used for the point‐of‐care assay of diverse biomarkers. Abstract : Metal–organic framework‐derived bimetallic Cu5 Zn8 hollow porous carbon nanocubes anchored gold nanoparticles (AuNPs) are used as signal labels and improve the surface area, and catalytic active sites to load the detection antibody. Electrodeposited AuNPs on nitrogen‐doped holey graphene are utilized as substrates to enhance the capture antibody immobilization and quicken the electron transfer rate for insulin detection.