Central nervous system sarcoma with ATXN1:DUX4 fusion expands the concept of CIC‐rearranged sarcoma. Issue 11 (13th July 2022)

• Record Type: Journal Article
• Authors: Satomi, Kaishi; Ohno, Makoto; Kubo, Takashi; Honda‐Kitahara, Mai; Matsushita, Yuko; Ichimura, Koichi; Narita, Yoshitaka; Ichikawa, Hitoshi; Yoshida, Akihiko
• Is Part Of: Genes, chromosomes & cancer. Volume 61:Issue 11(2022)
• Page Start: 683
• Page End: 688
• Publication Date: 2022-07-13
• Subjects: ATXN1 -- central nervous system -- CIC -- DUX4 -- sarcoma; Cancer -- Genetic aspects -- Periodicals; 616.994042
• DOI: 10.1002/gcc.23080
• Languages: English
• ISSNs: 1045-2257

Abstract

Abstract: CIC ‐rearranged sarcoma is a high‐grade sarcoma, most often harboring CIC::DUX4 fusion, and is characterized by a distinct round cell histology, co‐expression of ETV4 and WT1, and a specific DNA methylation class. Herein, we report a brain tumor with ATXN1::DUX4 that had an indistinguishable phenotype and DNA methylation profile from CIC ‐rearranged sarcoma. A 40‐year‐old man presented with a 5 cm hemorrhagic mass in the right frontal lobe of the cerebrum. The tumor was resected and histologically showed a dense proliferation of relatively monomorphic round cells with multifocal myxoid changes. Immunohistochemically, the tumor was diffusely positive for ETV4, WT1, and DUX4. Through classic histomorphology and immunoprofile, the tumor was provisionally diagnosed as CIC ‐rearranged sarcoma. However, no CIC fusions or mutations were identified using CIC break‐apart fluorescence in situ hybridization (FISH) or FoundationOne CDx. Despite multiple surgeries and adjuvant chemoradiation therapy, the patient succumbed 16 months after presentation. RNA exome sequencing detected an in‐frame intraexonic ATXN1 (exon 9) ::DUX4 (exon 1) fusion, which was validated by reverse transcription‐polymerase chain reaction and ATXN1 FISH assay. Upon DNA methylation analysis, the tumor matched with CIC ‐rearranged sarcoma both by the Deutsche Krebsforschungszentrum classifier and t‐distributed stochastic neighbor embedding. Along with a recent report of a similar pediatric brain tumor, the present case suggests that ATXN1::DUX4 is a recurrent alternative molecular event in the sarcoma type that is presently defined by CIC rearrangement, which prompts an expansion of the tumor concept.