Oxygen-powered flower-like FeMo6@CeO2 self-cascade nanozymes: a turn-on enhancement fluorescence sensor. Issue 34 (11th August 2022)

• Record Type: Journal Article
• Authors: Tong, Zhibo; Wang, Tong; Cai, Yunheng; Sha, Jingquan; Peng, Tai
• Is Part Of: Journal of materials chemistry. Volume 10:Issue 34(2022)
• Page Start: 6425
• Page End: 6432
• Publication Date: 2022-08-11
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb01466a
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A novel integrated nanozyme (FeMo6 @CeO2 ) is synthesized. It was found that the effect on the substrate adsorption capacity due to specific surface area was more significant than active site exposure for cascade mimetic enzyme activity. Abstract : Enzyme cascade reactions in organisms have sparked tremendous interest for their coupled catalysis-facilitated efficient biochemical reactions. However, multi-enzyme cascade nanozymes remain largely unused. In the work, flower-like porous ceria-based integrated enzymes (INAzyme), FeMo6 @CeO2 (FMC- n ), were readily prepared using an efficient thermally induced process. Owing to a larger specific surface area and excellent adsorption, the flower-like matrix (CeO2 ) can serve as not only an effective sorbent for the conversion of dissolved oxygen in solution but also an excellent support for self-cascade reactions with FeMo6 . Based on the electron transfer through Fe and Ce cycles, FMC- n INAzyme exhibits intrinsic oxidase-, peroxidase- and Fenton-like activities. Moreover, by assessing the Vo mobility and F-Vo relative content of FMC-2 and FC-66, we found that the contribution of enhanced accessibility through the specific surface area (SSA) to the activity of the INAzyme is significantly higher than the active sites. Finally, a fluorescence turn-on enhancement sensing platform based on self-cascade FMC- n for the detection of Cys was established, culminating in a LOD of as low as 0.014 μM in the range of 1–100 μM.