Glycomacropeptide from camel milk inhibits the adhesion of enterotoxigenic Escherichia coli K88 to porcine cells. (November 2022)

• Record Type: Journal Article
• Authors: Althnaibat, Rami M.; Koch, Mandy; Bruce, Heather L.; Wefers, Daniel; Gänzle, Michael G.
• Is Part Of: International dairy journal. Volume 134(2022)
• Publication Date: 2022-11
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2022.105448
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: Enterotoxigenic Escherichia coli (ETEC) are a major cause of childhood diarrhoea and of diarrhoea in piglets and calves. This study compared the anti-adhesive activities against ETEC of glycomacropeptide (GMP) from Bactrian camels and dromedaries with that of bovine GMP. GMP was purified by ion exchange chromatography and ultra-filtration. The monosaccharide content of GMP from Bactrian camels and dromedaries was about twice as high when compared with bovine GMP. Glycans from camels included fucose and N-acetylglucosamine, which were absent in bovine GMP. GMP from both camel species prevented ETEC adhesion to porcine blood cells at a concentration of 0.24 g L −1 to 0.28 g L −1 respectively, a concentration that is about 20-fold lower when compared with bovine GMP. This increased activity likely relates to the increased glycosylation and the density of glycan spacing, and/or to differences in the glycan composition.