Factors influencing mosaicism: a retrospective analysis. Issue 3 (September 2022)

• Record Type: Journal Article
• Authors: Huang, Qiu-Xiang; Wang, Zhi-Hong; Huang, Wu-Jian; Mao, Li-Hua; Lin, Chun-Li; Chen, Guo-Yong; Wang, Cai-Xia; Chen, Zhi-Biao; Lin, Yu-Lin; He, Ling-Yun; Liu, Yun
• Is Part Of: Reproductive biomedicine online. Volume 45:Issue 3(2022)
• Page Start: 491
• Page End: 500
• Publication Date: 2022-09
• Subjects: ART -- Fertilization method -- Mosaicism -- PGT-A -- Preimplantation genetic testing for aneuploidies -- Semen quality; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2022.04.020
• Languages: English
• ISSNs: 1472-6483

Abstract

Abstract: Research question: What factors affect the incidence of mosaic embryos resulting from assisted reproductive technology? Design: A retrospective analysis of data from preimplantation genetic testing for aneuploidies in 544 couples was conducted using data from an electronic medical record database. Results: Of 1910 embryos studied, 127 (6.6%) were mosaic. In multivariable logistic regression analysis, mosaicism incidence increased in embryos from IVF versus intracytoplasmic sperm injection (ICSI) (odds ratio [OR] 4.560, 95% confidence interval [CI] 2.800–7.424, P < 0.001), and in embryos from abnormal versus normal semen (OR 3.496, 95% CI 2.455–4.979, P < 0.001). Embryos tested using SurePlex 24Sure had lower mosaicism percentages than those tested using MALBAC-NGS and PicoPLEX GenetiSure (OR 2.726, 95% CI 1.532–4.852, P = 0.001; OR 2.389, 95% CI 1.537–3.711, P < 0.001, respectively). Conclusions: Semen quality, fertilization method and detection system are independent factors associated with embryonic mosaicism.