Plasma skeletal muscle myosin phenotypes identified by immunoblotting are associated with pulmonary embolism occurrence in young adults. Issue 189 (May 2020)

• Record Type: Journal Article
• Authors: Deguchi, Taichi K.; Deguchi, Hiroshi; Guo, Zihan; Elias, Darlene J.; Griffin, John H.
• Is Part Of: Thrombosis research. Issue 189(2020)
• Page Start: 88
• Page End: 92
• Publication Date: 2020-05
• Subjects: SkM skeletal muscle myosin -- HMM heavy meromyosin -- MW molecular weight -- VTE venous thromboembolism -- PE pulmonary embolism -- DVT deep vein thrombosis; Myosin -- Skeletal muscle myosin -- Venous thrombosis -- Pulmonary embolism -- Immunoblot -- Thrombin; Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2020.02.020
• Languages: English
• ISSNs: 0049-3848

Abstract

Abstract: Background: Purified skeletal muscle myosin (SkM) binds factor Xa and is procoagulant. The molecular forms of SkM in human plasma have not been characterized. Method: Human plasma SkM heavy chain (HC) isoforms of different molecular weights were detected by a newly developed immunoblotting protocol. In this pilot study, the distribution of SkM HC antigen isoforms in plasmas of healthy subjects and young adult patients with venous thrombosis was analyzed. Results: Multiple SkM HC antigen bands were detected in human plasmas, corresponding to full-length SkM HC, heavy meromyosin, or the S1 fragment. Plasma immunoblots of healthy subjects displayed three major phenotypes: Type I with two primary bands for full-length SkM and heavy meromyosin, and two lesser bands including S1 fragment (54%); Type II with bands primarily for full-length SkM HC (34%); and Type III with only a band for the S1 fragment (12%). Plasma SkM HC antigen Type II phenotype was associated with an increased occurrence of isolated pulmonary embolism in younger patients, respectively (≤50 years old). Conclusions: Three SkM HC antigen phenotypes were identified in human plasma by immunoblotting, and Type II phenotype was correlated with the occurrence of isolated pulmonary embolisms in younger patients. Highlights: Immunoblotting detects multiple skeletal muscle myosin (SkM) isoforms in human plasma. Plasma contains SkM heavy chain, heavy meromyosin, and subfragment-1-like fragments. Three principal phenotypes of SkM heavy chain antigen are found in human plasma. SkM phenotypes might be linked positively or negatively with pulmonary embolism.