THE ROLE OF DNA DOUBLE-STRAND BREAK REPAIR THROUGH NON-HOMOLOGOUS END JOINING IN THE DOSE-RATE EFFECT IN TERMS OF CLONOGENIC ABILITY. Issue 13 (9th September 2022)

• Record Type: Journal Article
• Authors: Tsuchiya, Hisayo; Shimada, Mikio; Tsukada, Kaima; Meng, Qingmei; Kobayashi, Junya; Matsumoto, Yoshihisa
• Is Part Of: Radiation protection dosimetry. Volume 198:Issue 13/15(2022)
• Page Start: 990
• Page End: 997
• Publication Date: 2022-09-09
• Subjects: Radiation dosimetry -- Periodicals; Radiation -- Safety measures -- Periodicals; 363.1799
• DOI: 10.1093/rpd/ncac030
• Languages: English
• ISSNs: 0144-8420

Abstract

Abstract: It is generally and widely accepted that the biological effects of a given dose of ionizing radiation, especially those of low linear energy transfer radiations like X-ray and gamma ray, become smaller as the dose rate becomes lower. This phenomenon, known as 'dose-rate effect (DRE), ' is considered due to the repair of sublethal damage during irradiation but the precise mechanisms for DRE have remained to be clarified. We recently showed that DRE in terms of clonogenic cell survival is diminished or even inversed in rodent cells lacking Ku, which is one of the essential factors in the repair of DNA double-strand breaks (DSBs) through non-homologous end joining (NHEJ). Here we review and discuss the involvement of NHEJ in DRE, which has potential implications in radiological protection and cancer therapeutics.