Single intratesticular injection of blood‐serum‐derived exosomes can potentially alleviate testopathy following testicular torsion. Issue 4 (20th May 2022)

• Record Type: Journal Article
• Authors: Keivan, Mona; Mansouri Torghabeh, Fatemeh; Davoodi, Samira; Moradi Maryamneghari, Shima; Dadfar, Reza
• Is Part Of: Animal models and experimental medicine. Volume 5:Issue 4(2022)
• Page Start: 362
• Page End: 368
• Publication Date: 2022-05-20
• Subjects: anti‐inflammation -- exosome -- parameter -- sperm -- testicular torsion; Laboratory animals -- Periodicals; Diseases -- Animal models -- Periodicals; Animal models in research -- Periodicals; Veterinary medicine -- Periodicals; Laboratory Animal Science; Disease Models, Animal; Animals, Laboratory; Animal Welfare; Veterinary Medicine; Animal models in research; Diseases -- Animal models; Laboratory animals; Veterinary medicine; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 616.0273
• DOI: 10.1002/ame2.12232
• Languages: English
• ISSNs: 2576-2095

Abstract

Abstract: Background: Testicular torsion (TT) is an acute inflammatory process leading to male infertility. Today, anti‐inflammatory effects of exosomes derived from blood serum are used in various laboratory procedures. In the present study, the anti‐inflammatory effects of blood‐serum‐derived exosomes in treatment of acute inflammation following TT in mice were evaluated. Materials and Methods: Eighteen male mice were grouped as healthy control, TT, and TT + exosome. TT was induced surgically, and exosomes were extracted from blood serum and administrated by a single intratesticular injection (10 IU). Malondialdehyde (MDA) and Griess assays were used to evaluate the level of oxidative stress. Sperm indices, testosterone (Tes), and apoptotic gene expression (p‐53, Bcl2, and Caspase‐3) were also assessed. H&E and immunohistochemistry (IHC) stainings were used for histopathological investigations. Data analysis was applied by SPSS (v.19) software. Results: Oxidative stress and apoptotic genes expression were increased significantly ( p < 0.05) in TT group compared with control. Sperm parameters and Tes were significantly increased, and expression of apoptotic genes was significantly reduced in TT + exosome group ( p < 0.05). Conclusion: Since the blood‐serum‐derived exosomes have anti‐inflammatory features, the intratesticular application of blood‐serum‐derived exosomes can be used clinically in acute phase of orchitis following TT to inhibit testicular inflammation. Abstract : Blood‐derived exosomes can act as anti‐inflammatory sources in TT. This study showed that administration of a single dose of exosome immediately after an acute testicular injury can significantly reduce TT complications. Our study showed that exosomes can diminish apoptosis and testicular inflammation and lead to the production of high‐quality sperm. It is suggested that in other experimental studies, the molecular effect of exosomes in the treatment of acute inflammation should be investigated to determine the apparent role of this substance. Thus, the exosome may be suggested as a treatment protocol for the relief of acute inflammation such as TT. For future investigations, it is recommended to use exosomes derived from other body fluids in TT pathologic condition.