Characteristics of gut microbiota in representative mice strains: Implications for biological research. Issue 4 (26th July 2022)

• Record Type: Journal Article
• Authors: Guo, Jianguo; Song, Chenchen; Liu, Yunbo; Wu, Xuying; Dong, Wei; Zhu, Hua; Xiang, Zhiguang; Qin, Chuan
• Is Part Of: Animal models and experimental medicine. Volume 5:Issue 4(2022)
• Page Start: 337
• Page End: 349
• Publication Date: 2022-07-26
• Subjects: Bacteroidetes -- BALB/c mice -- C57BL/6J -- disease‐related animal -- Firmicutes -- gene‐editing animal -- gut microbiota -- immunity defect animal -- strains; Laboratory animals -- Periodicals; Diseases -- Animal models -- Periodicals; Animal models in research -- Periodicals; Veterinary medicine -- Periodicals; Laboratory Animal Science; Disease Models, Animal; Animals, Laboratory; Animal Welfare; Veterinary Medicine; Animal models in research; Diseases -- Animal models; Laboratory animals; Veterinary medicine; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 616.0273
• DOI: 10.1002/ame2.12257
• Languages: English
• ISSNs: 2576-2095

Abstract

Abstract: Background: Experimental animals are used to study physiological phenomena, pathological mechanisms, and disease prevention. The gut microbiome is known as a potential confounding factor for inconsistent data from preclinical studies. Although many gut microbiome studies have been conducted in recent decades, few have focused on gut microbiota fluctuation among representative mouse strains. Methods: A range of frequently used mouse strains were selected from 34 isolation packages representing disease‐related animal (DRA), immunity defect animal (IDA), or gene‐editing animal (GEA) from the BALB/c and C57BL/6J backgrounds together with normal mice, and their microbial genomic DNA were isolated from mouse feces to sequence for the exploration of gut microbiota. Results: Mouse background strain, classification, introduced source, introduced year, and reproduction type significantly affected the gut microbiota structure ( p < 0.001 for all parameters), with background strain contributing the greatest influence ( R 2 = 0.237). In normal groups, distinct gut microbiota types existed in different mouse strains. Sixty‐four core operational taxonomic units were obtained from normal mice, and 12 belonged to Lactobacillus. Interestingly, the gut microbiota in C57BL/6J was more stable than that in BALB/c mice. Furthermore, the gut microbiota in the IDA, GEA, and DRA groups significantly differed from that in normal groups ( p < 0.001 for all). Compared with the normal group, there was a significantly higher Chao1 and Shannon index ( p < 0.001 for all) in the IDA, GEA, and DRA groups. Markedly changed classes occurred with Firmicutes and Bacteroidetes . The abundances of Helicobacter, Blautia, Enterobacter, Bacillus, Clostridioides, Paenibacillus, and Clostridiales all significantly decreased in the IDA, GEA, and DRA groups, whereas those of Saccharimonas, Rikenella, and Odoribacter all significantly increased. Abstract : Characteristics of gut microbiota in representative mice strains. Background Strain, classification, introduction source, introduction year and reproduction type had significant effects on gut flora structure of different strains of mice. In normal groups, distinct gut flora types existed in different strains. Sixty‐four core operational taxonomic units were obtained from normal mice. There were also significant differences in the intestinal bacteria of the mice in the immunity defect animal, gene‐editing animal and disease‐related animal.