Fluorescence spectral detection of copper(II), mercury(II) and zinc(II) based on a multifunctional peptide-based sensor. Issue 7 (9th August 2022)

• Record Type: Journal Article
• Authors: Zhang, Lianshun; Yu, Shuaibing; Gao, Lei; Meng, Wei; Fu, Chen; Li, Lianzhi
• Is Part Of: Spectroscopy letters. Volume 55:Issue 7(2022)
• Page Start: 488
• Page End: 499
• Publication Date: 2022-08-09
• Subjects: Copper(II) -- fluorescence spectroscopy -- mercury(II) -- peptide sensor -- zinc(II); Spectrum analysis -- Periodicals; 543.5
• DOI: 10.1080/00387010.2022.2101477
• Languages: English
• ISSNs: 0038-7010

Abstract

Abstract: A fluorescence peptide-based sensor (Dansyl-His-Thr-Glu-His-Trp-NH2, D-P5) was synthesized by Fmoc solid phase peptide synthesis, using dansyl group as the fluorophore. The sensor exhibited different fluorescence responses and sensitive detections for copper(II), mercury(II) and zinc(II) ions as a multifunctional sensor. The detailed investigation suggested that the limits of detection were 37.6 nmol⋅L −1, 37.8 nmol⋅L −1 and 59.4 nmol⋅L −1 for copper(II), mercury(II) and zinc(II) ions, respectively, and the highly sensitive sensor was not influenced by other species. The sensor exhibited a strong binding ability to copper(II), mercury(II) and zinc(II) ions with the binding constants of 3.23 × 10 5 L⋅mol −1, 6.37 × 10 5 L⋅mol −1 and 6.0 × 10 4 L⋅mol −1, respectively. The binding stoichiometries of the sensor to copper(II), mercury(II) and zinc(II) ions measured by Job's plot were all 1:1. Furthermore, The sensor has been successfully used for the detections of copper(II), mercury(II) and zinc(II) in actual water samples.