Treatment with human umbilical cord blood serum in a gentamicin-induced nephrotoxicity model in rats. (3rd September 2022)

• Record Type: Journal Article
• Authors: Mirazi, Naser; Baharvand, Fatemeh; Moghadasali, Reza; Nourian, Alireza; Hosseini, Abdolkarim
• Is Part Of: Drug and chemical toxicology. Volume 45:Number 5(2022)
• Page Start: 2262
• Page End: 2268
• Publication Date: 2022-09-03
• Subjects: Gentamicin -- nephrotoxicity -- umbilical cord blood serum -- rat -- inflammation; Toxicology -- Periodicals; Drugs -- Toxicology -- Periodicals; Toxicology, Experimental -- Periodicals; 615.9005
• DOI: 10.1080/01480545.2021.1926475
• Languages: English
• ISSNs: 0148-0545

Abstract

Abstract: Sold under the brand name of Garamycin, gentamicin (GM) is an antibiotic in the category of aminoglycoside, that although does have many antibacterial properties, owing to several side effects, its consumption is confined. The current study is aimed at gauging the protective influences of human umbilical cord blood serum (hUCBS) on nephrotoxicity which is induced by GM. In this regard, in the present experimental design, twenty-eight male Wistar rats with the weights of 220 ± 20 g were categorized randomly into 4 groups of seven. The groups included GM (100 mg/kg), control as well as hUCBS at doses of one and two percent together with GM (100 mg/kg) for ten days in an intraperitoneal manner. Blood sampling was collected from the heart directly 24 h after the final injection for obtaining blood serum; the parameters of C-reactive protein (CRP), total oxidant status (TOS), interleukin (IL)-6, lactate dehydrogenase (LDH), total antioxidant capacity (TAC), creatinine (Cr), blood urea nitrogen (BUN), blood serum glutathione (GSH) were gauged in blood serum samples to evaluate renal function. Moreover, for histology, an examination of kidney tissue was performed. In comparison to those of the GM group, in the treatment group, hUCBS significantly decreased the levels of BUN, Cr, LDH, TOS, IL-6, and the CRP levels, and significantly increased the TAC and GSH levels. It was revealed that the treatment of the animals with hUCBS culminates in the reduction of GM' toxic impacts on the kidney.