Exogenous hydrogen sulfide mediates Na+ and K+ fluxes of salt gland in salt-secreting mangrove plant Avicennia marina. (12th April 2022)

• Record Type: Journal Article
• Authors: Wei, Ming-Yue; Li, Huan; Zhang, Lu-Dan; Guo, Ze-Jun; Liu, Ji-Yun; Ding, Qian-Su; Zhong, You-Hui; Li, Jing; Ma, Dong-Na; Zheng, Hai-Lei
• Editors: Ball, Marilyn
• Is Part Of: Tree physiology. Volume 42:Number 9(2022)
• Page Start: 1812
• Page End: 1826
• Publication Date: 2022-04-12
• Subjects: Na+/H+ antiporter -- H+-ATPase -- Na+ and K+ fluxes -- hydrogen sulfide -- mangrove -- salt gland; Trees -- Physiology -- Periodicals; 582.16
• DOI: 10.1093/treephys/tpac042
• Languages: English
• ISSNs: 0829-318X

Abstract

Abstract: Hydrogen sulfide (H2 S), is a crucial biological player in plants. Here, we primarily explored the interaction between sodium hydrosulfide (NaHS, a H2 S donor) and the fluxes of Na + and K + from the salt glands of mangrove species Avicennia marina (Forsk.) Vierh. with non-invasive micro-test technology (NMT) and quantitative real-time PCR (qRT-PCR) approaches under salinity treatments. The results showed that under 400-mM NaCl treatment, the addition of 200-μM NaHS markedly increased the quantity of salt crystals in the adaxial epidermis of A. marina leaves, accompanied by an increase in the K + /Na + ratio. Meanwhile, the endogenous content of H2 S was dramatically elevated in this process. The NMT result revealed that the Na + efflux was increased from salt glands, whereas K + efflux was decreased with NaHS application. On the contrary, the effects of NaHS were reversed by H2 S scavenger hypotaurine (HT), and DL-propargylglycine (PAG), an inhibitor of cystathionine-γ-lyase (CES, a H2 S synthase). Moreover, enzymic assay revealed that NaHS increased the activities of plasma membrane and tonoplast H + -ATPase. qRT-PCR analysis revealed that NaHS significantly increased the genes transcript levels of tonoplast Na + /H + antiporter ( NHX1 ), plasma membrane Na + /H + antiporter ( SOS1 ), plasma membrane H + -ATPase ( AHA1 ) and tonoplast H + -ATPase subunit c ( VHA-c1 ), while suppressed above-mentioned gene expressions by the application of HT and PAG. Overall, H2 S promotes Na + secretion from the salt glands of A. marina by up-regulating the plasma membrane and tonoplast Na + /H + antiporter and H + -ATPase.