APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome . (12th January 2022)

• Record Type: Journal Article
• Authors: Khalfi, Pierre; Suspène, Rodolphe; Caval, Vincent; Thiers, Valérie; Beauclair, Guillaume; Marchio, Agnès; Bekondi, Claudine; Amougou Atsama, Marie; Camengo-Police, Serge Magloire; Noah Noah, Dominique; Njouom, Richard; Blanc, Hervé; Vallet, Thomas; Vignuzzi, Marco; Pineau, Pascal; Vartanian, Jean Pierre
• Is Part Of: Journal of infectious diseases. Volume 226:Number 5(2022)
• Page Start: 891
• Page End: 895
• Publication Date: 2022-01-12
• Subjects: Africa -- APOBEC3C -- editing -- hepatitis B virus -- nucleotide context -- patients -- polymorphism; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiac003
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA processivity of the enzyme. In this study, we demonstrated in culture and in infected patients that hepatitis B virus (HBV) could be edited by APOBEC3CS188I . Using next-generation sequencing, we demonstrated that APOBEC3CS188I led to enhanced editing activity in 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of this enzyme, which could be used to determine its impact on HBV or nuclear DNA. Abstract : In this study, we demonstrated in culture and in patients that HBV could be edited by APOBEC3CS188I . This enzyme led to an enhanced editing activity in a more specific 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of APOBEC3CS188I .