Label-free visualization of cellular response to molecularly targeted agents using multiplex coherent anti-Stokes Raman scattering and third harmonic generation microscopy. (1st October 2022)

• Record Type: Journal Article
• Authors: Oka, Yuki; Shen, Larina Tzu-Wei; Mori, Tomoko; Iwamura, Takumi; Leproux, Philippe; Matsusaka, Satoshi; Kano, Hideaki
• Is Part Of: Applied physics express. Volume 15:Number 10(2022)
• Publication Date: 2022-10-01
• Subjects: Raman -- CARS -- spectroscopy -- microscopy -- imaging; Physics -- Periodicals; Technology -- Periodicals; 621.05
• DOI: 10.35848/1882-0786/ac8d47
• Languages: English
• ISSNs: 1882-0778

Abstract

Abstract: Cellular responses to molecularly targeted agents (gefitinib and osimertinib) have been observed in cultured cancer cells using ultra-broadband multiplex coherent anti-Stokes Raman scattering and third harmonic generation microscopy. Lipid droplets (LDs) appeared in the cells 48 h after the exposure of cancer cells to gefitinib. This was also observed in the gefitinib-resistant strain, and the accumulation of LDs was significant. When exposed to osimertinib, LDs were not as pronounced as those with gefitinib. These agents act on cells through different mechanisms.