Identifying and Differentiating Topological G‐Quadruplex Structures with DNA‐Encoded Plasmonic Gold Nanoparticles. (19th August 2022)

• Record Type: Journal Article
• Authors: Song, Tingjie; Wang, Xiaojing; Yao, Dongbao; Liang, Haojun; Lu, Yi
• Is Part Of: Angewandte Chemie. Volume 134:Number 39(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-08-19
• Subjects: DNA -- Extinction -- G-Quadruplex -- Plasmonic Nanoparticle -- Topology; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202204201
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: DNA G‐quadruplexes (G4s) have been identified as critical elements in modulating genomic functions and many other biological processes. Their functions are highly dependent on the primary nucleotides and secondary folding structures. Therefore, to understand their functions, methods to identify and differentiate structures of G4 with speed and accuracy are required but limited. In this report, we have applied a synthetic G4 DNA‐encoded nanoparticle approach to identify and differentiate G4 DNA molecules with different topologies and nucleotide residues. We found that the resulting plasmonic properties of the gold nanoparticles, monitored by UV/Vis spectroscopy, are quite sensitive to different G4 structures, including stacking layers, loop sequences, capping bases on G4s, and topological structures. Through these systematic investigations, we demonstrate that this G4‐encoded gold nanoparticle approach can be used to profile the G4 structures and distinguish G4s from human telomeres. Such a method may have wide applications in G4 research. Abstract : The G‐quadruplex (G4) structures are resolved with a DNA‐encoded nanoparticle growth strategy. In this method, through monitoring the UV/Vis extinction of plasmonic gold nanoparticles grown in the presence of G4s, both topological structures and nucleotide residues within G4 DNA, are identified and differentiated.