The Inhibitory Effect of Blue Light on Phagocytic Activity by ARPE‐19 Cells. (3rd February 2022)

• Record Type: Journal Article
• Authors: Olchawa, Magdalena M.; Herrnreiter, Anja M.; Skumatz, Christine M.B.; Krzysztynska‐Kuleta, Olga I.; Mokrzynski, Krystian T.; Burke, Janice M.; Sarna, Tadeusz J.
• Is Part Of: Photochemistry and photobiology. Volume 98:Number 5(2022)
• Page Start: 1110
• Page End: 1121
• Publication Date: 2022-02-03
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.13596
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: Chronic exposure of the retina to short wavelength visible light is a risk factor in pathogenesis of age‐related macular degeneration. The proper functioning and survival of photoreceptors depends on efficient phagocytosis of photoreceptor outer segments (POS) by retinal pigment epithelium. The purpose of this study was to analyze the phagocytic activity of blue light–treated ARPE‐19 cells, and to examine whether the observed effects could be related to altered levels of POS phagocytosis receptor proteins and/or to oxidation of cellular proteins and lipids. POS phagocytosis was measured by flow cytometry. Phagocytosis receptor proteins αv and β5 integrin subunits and Mer tyrosine kinase (MerTK) were quantified by western blotting. The intact functional heterodimer αvβ5 was quantified by immunoprecipitation followed by immunoblotting. Cellular protein and lipid hydroperoxides were analyzed by coumarin boronic acid probe and iodometric assay, respectively. Cell irradiation induced reversible inhibition of specific phagocytosis and transient reductions in phagocytosis receptor proteins. Full recovery of functional heterodimer was apparent. Significant photooxidation of cellular proteins and lipids was observed. The results indicate that transient inhibition of specific phagocytosis by blue light could be related to the reduction in phagocytosis receptor proteins. Such changes may arise from oxidative modifications of cell phagocytic machinery components. Abstract : Long‐term exposure to short wavelength visible light was defined as a risk factor in development of age‐related macular degeneration (AMD). The purpose of this study was to analyze the phagocytic activity of blue light–treated ARPE‐19 cells, and to examine whether the observed effects could be related to altered levels of POS phagocytosis receptor proteins and to oxidation of cellular proteins and lipids. The results indicate that inhibition of the specific phagocytosis of RPE cells by blue light could be related to the reduction in phagocytosis receptor proteins, as well as the dose‐dependent photooxidation of cellular proteins, and unsaturated membrane lipids.