Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine. Issue 3 (9th August 2022)

• Record Type: Journal Article
• Authors: Cahoon, Laty A.; Alejandro‐Navarreto, Xiomarie; Gururaja, Avinash N.; Light, Sam H.; Alonzo, Francis; Anderson, Wayne F.; Freitag, Nancy E.
• Is Part Of: Molecular microbiology. Volume 118:Issue 3(2022)
• Page Start: 278
• Page End: 293
• Publication Date: 2022-08-09
• Subjects: chaperone -- listeria -- PPIase -- PrsA1 -- PrsA2 -- secretion; Molecular microbiology -- Periodicals; 572.829
• DOI: 10.1111/mmi.14967
• Languages: English
• ISSNs: 0950-382X

Abstract

Abstract: Listeria monocytogenes ( Lm ) is a widespread environmental Gram‐positive bacterium that can transition into a pathogen following ingestion by a susceptible host. To cross host barriers and establish infection, Lm is dependent upon the regulated secretion and activity of many proteins including PrsA2, a peptidyl‐prolyl cis‐trans isomerase with foldase activity. PrsA2 contributes to the stability and activity of a number of secreted virulence factors that are required for Lm invasion, replication, and cell‐to‐cell spread within the infected host. In contrast, a second related secretion chaperone, PrsA1, has thus far no identified contributions to Lm pathogenesis. Here we describe the characterization of a two‐component signal transduction system PieRS that regulates the expression of a regulon that includes the secretion chaperones PrsA1 and PrsA2. PieRS regulated gene products are required for bacterial resistance to ethanol exposure and are important for bacterial survival during transit through the gastrointestinal tract. PrsA1 was also found to make a unique contribution to Lm survival in the GI tract, revealing for the first time a non‐overlapping requirement for both secretion chaperones PrsA1 and PrsA2 during the process of intra‐gastric infection. Abstract : Listeria monocytogenes is an environmental bacterial pathogen that depends upon secreted proteins for survival within the infected host; proper folding of these proteins requires the secretion chaperone PrsA2. Described here is the characterization of a two‐component signal transduction system PieRS that regulates prsA2 expression as well as an additional chaperone and genes of unknown function. The PieRS regulon confers bacterial resistance to ethanol exposure and contributes to bacterial survival during transit through the gastrointestinal tract.