Efficient Imidazolium‐Biomolecule Interaction‐Assisted Amplified Quenching for Ultrasensitive Detection of Heparin. Issue 18 (13th July 2022)

• Record Type: Journal Article
• Authors: Lee, Seung Yeob; Song, Hyebin; Lee, Sun Woo; Han, Minwoo; Choi, Haemin; Lee, Seoung Ho
• Is Part Of: Chemistry, an Asian journal. Volume 17:Issue 18(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-07-13
• Subjects: self-assembly -- heparin -- amplified quenching -- imidazolium -- fluorescent sensors; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.202200458
• Languages: English
• ISSNs: 1861-4728

Abstract

Abstract: Detection of heparin (HP) under physiological conditions is difficult due to the presence of biological obstructions including proteins and lipids. Thus, it is highly challenging to selectively detect HP and to increase its sensitivity in complex systems. Here, we report the detection of HP at nanomolar levels via efficient imidazolium‐HP interaction‐assisted fluorescence quenching amplification. The self‐assembled pyrenyl aggregates are devised as a conduit for efficient exciton transport, which induces amplified fluorescence quenching for HP detection. This amplified quenching is enhanced by introducing an imidazolium receptor designed to have a high affinity to HP via electrostatic and/or additional interactions with C2 protons, resulting in a very high Stern‐Volmer quenching constant of approximately 1.17×10 8 M −1 . Abstract : A novel self‐assembled fluorescent sensor has been devised by introducing imidazolium capable of inducing a strong interaction with HP, in order to increase sensitivity. The enhanced affinity of the probe for HP exhibited improved amplified quenching effect with a very high SV quenching constant of K SV =1.17×10 8 M −1 .