Construction of DNA ligase-mimicking nanozymes via molecular imprinting. Issue 35 (8th February 2022)

• Record Type: Journal Article
• Authors: He, Xinpei; Luo, Qi; Guo, Zhanchen; Li, Ying; Liu, Zhen
• Is Part Of: Journal of materials chemistry. Volume 10:Issue 35(2022)
• Page Start: 6716
• Page End: 6723
• Publication Date: 2022-02-08
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d1tb02325j
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A strategy called affinity gathering-enhanced coupling and thermal cycling amplification is reported for designing enzyme-mimicking molecularly imprinted polymers. It is verified with mesoporous silica nanoparticles capable of ligating short ssDNA. Abstract : Enzyme mimics are of significant importance due to their facile preparation, low cost and stability to rigorous environments. Molecularly imprinted polymers (MIPs) have been important synthetic mimics of enzymes. However, effective strategies for the rational design of enzyme-mimicking MIPs have still remained limited. Herein, we report a new strategy, termed affinity gathering-enhanced coupling and thermal cycling amplification (AGEC-TCA), for the rational design and engineering of molecularly imprinted mesoporous silica nanoparticles (MSNs) that are capable of ligating short ssDNA fragments. This strategy relied on enhancing the effective collision probability via binding substrates into highly favorable orientation by product-imprinted MSNs as well as product release via thermal cycling which enabled successive product amplification. Using modified and natural hexadeoxyribonucleotide as templates, the prepared product-imprinted MSNs exhibited a remarkably enhanced reaction speed (by up to 63-fold) as well as excellent sequence specificity towards substrate trideoxyribonucleotides. Thus, this strategy opened up a new avenue to access enzyme mimics via molecular imprinting.