Engineering a DNA origami mediated multicolour quantum dot platform for sub-diffraction spectral separation imaging. Issue 37 (22nd August 2022)

• Record Type: Journal Article
• Authors: Huang, Da; Haddad, Lucy; Rahman, Fahmida; Palma, Matteo; Sapelkin, Andrei
• Is Part Of: RSC advances. Volume 12:Issue 37(2022)
• Page Start: 23778
• Page End: 23785
• Publication Date: 2022-08-22
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra04316e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The validation of super-resolution optical imaging techniques requires well-defined reference samples that can be used repeatedly and reliably as model standards. Abstract : The validation of super-resolution optical imaging techniques requires well-defined reference samples that can be used repeatedly and reliably as model standards. Here, we engineer a DNA origami scaffold-mediated multicolour quantum dot hybrid nanostructure and test it using a recently proposed Quantum Dot-based spectral separation technique. We show that multivalent DNA structures offer a robust and precise nanoscale quantum dot placement scaffold, while the spectral resolution method provides relatively simple and fast image acquisition capabilities using any standard confocal or fluorescence microscope capable of spectral signal separation and a single excitation laser wavelength.