Post‐anthesis saline‐alkali stress inhibits grain filling by promoting ethylene production and signal transduction. (15th May 2022)

• Record Type: Journal Article
• Authors: Peng, Yaqiong; Chen, Yinke; Yuan, Youming; Liu, Bohan; Yu, Peng; Song, Shihao; Yi, Yake; Teng, Zhenning; Yi, Zhenxie; Zhang, Jianhua; Meng, Shuan; Ye, Nenghui; Duan, Meijuan
• Is Part Of: Food and energy security. Volume 11:Number 3(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-05-15
• Subjects: ethylene -- grain filling -- rice -- Saline‐alkali stress -- starch accumulation; Climatic changes -- Periodicals; Crop improvement -- Periodicals; Food security -- Periodicals; Energy security -- Periodicals; Biology -- Periodicals; 333.9505
• DOI: 10.1002/fes3.384
• Languages: English
• ISSNs: 2048-3694

Abstract

Abstract: Saline‐alkali stress is one of the major abiotic stresses that severely affect rice yield. However, the mechanism by which saline‐alkali stress regulates grain filling in rice is still unclear. In this study, Oryza sativa L. spp. Indica cultivar Chaoyou1000 (C1000) was exposed to post‐anthesis saline‐alkali conditions at 6 days after anthesis, which significantly reduced the grain weight by suppressing the accumulation of starch and non‐structural carbohydrates in grains. Further analysis found that 1‐aminocyclopropane‐1‐carboxylate (ACC), a precursor for ethylene, was increased by saline‐alkali treatment. qRT‐PCR results showed that several key genes involved in ethylene biosynthesis, including the OsACS and OsACO genes, were upregulated in saline‐alkali‐treated grains. In addition, genes involved in the ethylene signalling pathway were also induced by saline‐alkali stress. Exogenous ethylene application reduced grain weight and both starch and NSC contents in grains of C1000, suggesting that saline‐alkali‐induced ethylene has a negative effect on grain filling. Furthermore, the gene expression levels of OsSUS, OsAGPL, OsAGPS, OsSSI and OsSSIIIa, which are key genes in the starch biosynthesis pathway, were downregulated in saline‐alkali‐treated grains. In agreement, assays on these enzymes further revealed that saline‐alkali stress decreased the activities of sucrose synthase (SUS), adenosine diphosphate glucose pyrophosphorylase (AGP) and starch synthase (StS). Together, our results indicated that saline‐alkali stress suppressed the enzyme activities involved in the conversion of sucrose to starch by elevating ethylene production, which led to inhibition of grain filling. Abstract : Post‐anthesis saline‐alkali stress increases ethylene production by upregulating gene expression of ethylene biosynthesis, which thus activates ethylene signalling transduction in rice grains. Gene expression and enzyme activities for starch accumulation in rice grains are both decreased by elevated ethylene, resulting in decreased efficiency of starch biosynthesis and eventually lower grain weight.