Pseudocitrobacter corydidari sp. nov., isolated from the Asian emerald cockroach Corydidarum magnifica. Issue 8 (23rd August 2022)

• Record Type: Journal Article
• Authors: Guzman, Juan; Poehlein, Anja; Glaeser, Stefanie P.; Schwengers, Oliver; Blom, Jochen; Hollensteiner, Jacqueline; Kämpfer, Peter; Vilcinskas, Andreas
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-23
• Subjects: cockroach microbiome -- Corydidarum magnifica -- Enterobacteriaceae -- Pseudocitrobacter corydidari; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005497
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-negative bacterial strain, G163CM T, was isolated from the gut of the Asian emerald cockroach Corydidarum magnifica . The 16S rRNA gene sequence (1416 bp) of strain G163CM T showed 99.22% similarity to Pseudocitrobacter faecalis CCM 8479 T and Pseudocitrobacter vendiensis CPO20170097 T . The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values of strain G163CM T were 92.4, 48.8 and 95.7% to P. faecalis CCM 8479 T, and 93.3, 52.4 and 95.7% to P. vendiensis CPO20170097 T . This strongly supports the designation of G163CM T as representing a new species in the genus Pseudocitrobacter . Phylogenetic trees based on the alignment of 16S rRNA, multilocus sequence analysis of six single-copy genes ( fusA, pyrG, leuS, rpoB, recN and mnmE ) and 107 core protein sequences consistently showed G163CM T to be a member of the genus Pseudocitrobacter, closely related to P. vendiensis CPO20170097 T . In contrast to P. faecalis CCM 8479 T and P. vendiensis CPO20170097 T, the genome of G163CM T did not encode for proteins conferring resistance to antibiotics. However, all three genomes encoded a similar number of virulence factors and specialized metabolite biosynthetic proteins. The major fatty acids of strain G163CM T were C16:0 (31.5 %), C18:1 ω7c (22.6 %), C17:0 cyclo (15.3 %) and C14:0 (6.5 %). Based on the polyphasic results, we conclude that strain G163CM T represents a novel species of the genus Pseudocitrobacter and we propose the name Pseudocitrobacter corydidari sp. nov. with the type strain G163CM T (=DSM 112648 T =CCM 9160 T ).