Actinomadura spongiicola sp. nov., isolated from the marine sponge Leucetta chagosensis. Issue 8 (24th August 2022)

• Record Type: Journal Article
• Authors: Qin, Qi; Jiang, Lin; Zhang, Die; Li, Lei; Lin, Hou-wen
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-24
• Subjects: Actinomadura spongiicola -- marine actinomycetes -- marine sponge -- new species; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005506
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel actinomycete strain, named LHW52907 T, was isolated from a marine sponge ( Leucetta chagosensis ) collected in the South China Sea. The strain developed branched mycelia without fragmentation and short spore chains in hook-and- spiral form with wrinkled surfaces, bearing no more 10 spores. The cell-wall hydrolysates contained meso -diaminopimelic acid as the diagnostic diamino acid. The sugars in whole-cell hydrolysates consisted of mannose, ribose, glucose, galactose and madurose. The major fatty acids of the strain were C16 : 0, C17 : 0 and C18 : 1 ω 9 c . The predominant menaquinone was MK-9(H6 ). The strain had the highest 16S rRNA gene sequence similarity of 99.72 % to Actinomadura pelletieri DSM 43383 T . However, the average nucleotide identity and in silico DNA–DNA hybridization values between them were 93.6 and 52.6 %, respectively, readily distinguishing them as two different species. The results indicate that strain LHW52907 T represents a novel species of the genus Actinomadura, for which we propose the name Actinomadura spongiicola sp. nov, with the type strain LHW52907 T (=DSM 106571 T =CGMCC 4.7596 T ).