Cohnella herbarum sp. nov., isolated from wild grass fermentation broth. Issue 8 (12th August 2022)

• Record Type: Journal Article
• Authors: Won, Miyoung; Lee, Shin Ae; Hong, Seung-Beom; Han, Byeong-Hak; Kwon, Soon-Wo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-12
• Subjects: Cohnella herbarum sp. nov. -- fermented liquor -- polyphasic taxonomic -- wild grasses -- 16S rRNA gene; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005485
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel strictly aerobic, Gram-stain-positive, rod-shaped, motile, endospore-forming, white-coloured bacterium, designated strain MFER-1 T, was isolated from a fermented liquor of wild grasses sampled in the Republic of Korea. The respiratory quinone of strain MFER-1 T was menaquinone-7 and its major cellular fatty acids were anteiso-C15 : 0 (55.3 %), iso-C16 : 0 (17.5 %) and C16 : 0 (12.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unidentified aminophospholipids and an unidentified phospholipid. The 16S rRNA gene sequence of strain MFER-1 T showed similarity of 98.1 % to ' Cohnella cholangitidis ' 1 605-214 T and below 98.0 % sequence similarity to the other Cohnella species. The phylogenomic tree indicated that strain MFER-1 T formed a reliable cluster with several Cohnella species. The estimated genome size of strain MFER-1 T was 8.52 Mb. Genomic DNA G+C content was 50.7mol%. The orthologous average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values of strain MFER-1 T with the most closely related species ' Cohnella cholangitidis ' 1 605-214 T were 78.7, 23.0 and 79.6 %, respectively. Based on the phenotypic, chemotaxonomic and phylogenetic results, strain MFER-1 T should represent a novel species of the genus Cohnella, for which the name Cohnella herbarum sp. nov. is proposed, with strain MFER-1 T (=KACC 21 257 T =NBRC 114 628 T ) as the type strain.