Desulfoferrobacter suflitae gen. nov., sp. nov., a novel sulphate-reducing bacterium in the Deltaproteobacteria capable of autotrophic growth with hydrogen or elemental iron. Issue 8 (12th August 2022)

• Record Type: Journal Article
• Authors: Davidova, Irene A.; Duncan, Kathleen E.; Wiley, Graham; Najar, Fares Z.
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-12
• Subjects: elemental iron (Fe0) -- Deltaproteobacteria -- Pseudomanadota -- sulphate-reducing bacteria -- Syntrophobacteraceae; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005483
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A mesophilic sulphate-reducing micro-organism, able to grow chemolithoautotrophically with H2 /CO2 (20 : 80) and with elemental iron as a sole electron donor, was isolated from a consortium capable of degrading long-chain paraffins and designated strain DRH4 T . Cells were oval shaped often with bright refractile cores and occurred singly or in pairs. The cells formed pili. Strain DRH4 T could grow chemolithoautotrophically with H2 /CO2 or elemental iron and chemoorganotrophically utilizing a number of organic substrates, such as fatty acids from formate to octanoate (C1 –C8 ). Sulphate and thiosulphate served as terminal electron acceptors, but sulphite and nitrate did not. Optimal growth was observed from 37 to 40 °C and pH from 6.5 to 7.2. Strain DRH4 T did not require NaCl for growth and could proliferate under a broad range of salinities from freshwater (1 g l −1 NaCl) to seawater (27 g l −1 NaCl) conditions. The genomic DNA G+C content was 54.46 mol %. Based on 16S rRNA gene sequence analysis. strain DRH4 T was distinct from previously described Deltaproteobacteria species exhibiting the closest affiliation to Desulforhabdus amnigena ASRB1 T, Syntrophobacterium sulfatireducens TB8106 T and Desulfovirga adipica 12016 T with 93.35, 93.42 and 92.85 % similarity, respectively. Strain DRH4 T showed significant physiological differences with the aforementioned organisms. Based on physiological differences and phylogenetic comparisons, we propose to classify DRH4 T as the type strain (=DSM 113 455 T =JCM 39 248 T ) of a novel species of a new genus with the name Desulfoferrobacter suflitae gen. nov., sp. nov.