Croceicoccus hydrothermalis sp. nov., isolated from shallow-sea hydrothermal system off Kueishantao Island. Issue 8 (8th August 2022)

• Record Type: Journal Article
• Authors: Chen, Beihan; Ye, Youting; Lin, Dan; Zhang, Mu; Sun, Jia; Tang, Kai
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-08
• Subjects: Croceicoccus hydrothermalis -- Kueishantao Island -- polyphasic taxonomic approach; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005472
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, strictly aerobic, non-motile, ovoid or short-rod shaped, orange-pigmented bacterial strain, designated as strain JLT1 T, was isolated from seawater of the shallow-sea hydrothermal system, near Kueishantao Islet. Growth was observed at 5–45°C (optimum, 30 °C) and pH 5.0–11.0 (optimum, pH 7.0). The salinity range for growth was 0–12 % (optimum, 2–4 %) (w/v) NaCl. JLT1 T contained ubiquinone-10 as the main respiratory quinone. Iso-C12 : 0, summed feature 3 (C16 : 1 ω7 c /ω6 c ) and summed feature 8 (C18 : 1 ω6 c /ω7 c ) were identified as the major cellular fatty acids. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, eight unidentified glycolipids and an unidentified lipid. The 16S rRNA gene of JLT1 T shared the greatest similarity (96.31 %) with those of Croceicoccus pelagius Ery9 T and Croceicoccus ponticola GM-16 T . The draft genome size of JLT1 T is 3.56 Mb, with 3578 potential genes and a genomic DNA G+C content of 63.24 mol %. Average nucleotide identity and digital DNA–DNA hybridization values of JLT1 T compared with C. pelagius Ery9 T, C. ponticola GM-16 T, Croceicoccus sediminis S2-4-2 T, Croceicoccus mobilis Ery22 T and Croceicoccus marinus E4A9 T were 74.5, 73.9, 74.4, 74.3 and 74.8 % and 20.6, 19.2, 20.0, 20.5 and 19.8%, respectively. On the basis of these phylogenetic, chemotaxonomic and phenotypic features, JLT1 T is concluded to represent a novel species of the genus Croceicoccus, for which the name Croceicoccus hydrothermalis sp. nov. is proposed. The type strain is JLT1 T (=CGMCC 1.15786 T =JCM 31508 T ).