Flavobacterium litorale sp. nov., isolated from red alga. Issue 8 (11th August 2022)

• Record Type: Journal Article
• Authors: Muhammad, Neak; Le Han, Ho; Lee, Yong-Jae; Ko, Jaeho; Nguyen, Tra T. H.; Kim, Song-Gun
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-11
• Subjects: Bacteroidota -- bacteria -- seaweed; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005458
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative and rod-shaped bacterial strain (WSW3-B6 T ) was isolated from red alga collected from the West Sea, Republic of Korea. Cells of strain WSW3-B6 T were non-motile, aerobic and produced slightly yellow and mucoid colonies on marine agar. The strain grew optimally at 23–30 °C, with 0.5–4 % NaCl (w/v) and at pH 6.5–8.5. A phylogenetic analysis of the 16S rRNA gene revealed that strain WSW3-B6 T belongs to the genus Flavobacterium within the family Flavobacteriaceae, having the highest sequence similarity to Flavobacterium arcticum SM1502 T (96.7%), followed by Flavobacterium salilacus subsp. altitudinum LaA7.5 T (96.2%) and Flavobacterium salilacus subsp. salilacus SaA2.12 T (96.2%). The complete sequence of a circular chromosome of strain WSW3-B6 T determined by combination of Oxford Nanopore and Illumina platforms comprised a total 2 725 095 bp with G+C content of 37.1 mol%. A comparative analysis based on the whole genome also showed the distinctiveness of strain WSW3-B6 T . The average nucleotide identity (ANI) values between strain WSW3-B6 T and the closest strains F. arcticum SM1502 T, F. salilacus subsp. altitudinum LaA7.5 T and F. salilacus subsp. salilacus SaA2.12 T were 78.3, 77.8 and 77.7 %, respectively, while the digital DNA–DNA hybridization (dDDH) values between strain WSW3-B6 T and the above closely related strains were 21.0, 20.4 and 20.3 %, respectively. Both the ANI and dDDH values supported the creation of a new species in the genus Flavobacterium . The major fatty acids (>10 %) were iso-C15 : 0 (19.3 %), C16 : 0 (14.0 %), iso-C17 : 0 3-OH (13.1 %) and C18 : 0 (10.7 %). The polar lipids of strain WSW3-B6 T included phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. Moreover, MK-6 was the only respiratory quinone. A comparison of the phylogenetic distinctiveness and the unique phenotypic and chemotaxonomic characteristics among strain WSW3-B6 T and closely related type strains supported that strain WSW3-B6 T (=KCTC 82708 T =GDMCC 1.2627 T ) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium litorale sp. nov. is proposed.