Parasphingorhabdus cellanae sp. nov., isolated from the gut of a Korean limpet, Cellana toreuma. Issue 8 (5th August 2022)

• Record Type: Journal Article
• Authors: Yoo, Ji-Ho; Han, Jeong Eun; Lee, June-Young; Jeong, Su-Won; Jeong, Yun-Seok; Lee, Jae-Yun; Lee, So-Yeon; Sung, Hojun; Tak, Euon Jung; Kim, Hyun Sik; Kim, Pil Soo; Choi, Jee-Won; Kim, Do-Yeon; Jeong, In Chul; Gim, Do-Hun; Kang, Seo Min; Bae, Jin-Woo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 8(2022)
• Publication Date: 2022-08-05
• Subjects: Cellana toreuma -- gut microbiota -- Korean seawater clam -- Parasphingorhabdus; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005470
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel bacterium, designated strain JHSY0214 T, was isolated from the gut of a Korean limpet, Cellana toreuma . Cells of strain JHSY0214 T were Gram-stain-negative, strictly aerobic, yellow-pigmented, non-spore-forming, non-motile and showed a rod–coccus growth cycle. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Parasphingorhabdus, and was most closely related to Parasphingorhabdus litoris KCTC 12764 T (98.71 %). Strain JHSY0214 T had two fluoroquinolone-resistance genes and seven multidrug-resistance efflux pump genes, but did not have beta-lactamase genes and zinc resistance genes compared with P. litoris KCTC 12764 T . Strain JHSY0214 T grew optimally at 30 °C, pH 7.0 and in the presence of 2 % (w/v) NaCl. The predominant cellular fatty acids of strain JHSY0214 T were summed feature 8 (C18 : 1 ω 6 c and/or C18 : 1 ω 7 c ; 41.2 %), summed feature 3 (C16 : 1 ω 7 c and/or C16 : 1 ω 6 c ; 21 %) and C16 : 0 (18.9 %). The major isoprenoid quinone was ubiquinone-10. The major polar lipids were sphingoglycolipid and phosphatidylethanolamine. The genomic DNA G+C content was 52.8 mol%. Based on phylogenetic, genotypic and phenotypic data, strain JHSY0214 T represents a novel species of the genus Parasphingorhabdus, for which the name Parasphingorhabdus cellanae is proposed. The type strain is JHSY0214 T (=KCTC 82387 T =DSM 112279 T ).