Development and validation of an HILIC–MS/MS method by one-step precipitation for chloroquine in miniature pig plasma. (June 2016)

• Record Type: Journal Article
• Authors: Wang, Zhan-Zhang; Lu, Hao-Yang; Shang, De-Wei; Ni, Xiao-Jia; Zhang, Ming; Wen, Yu-Guan
• Is Part Of: Bioanalysis. Volume 8:Number 11(2016)
• Page Start: 1159
• Page End: 1171
• Publication Date: 2016-06
• Subjects: chloroquine -- HILIC -- LC–MS/MS; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2015-0032
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Quantification of polar compounds such as chloroquine by revered-phase LC is a challenge because of poor retention and silanol interactions with stationary phase. Strong ion-pairing reagents added to mobile phases to improve reversed-phase retention and improve peak shape can be harmful for MS.Results: This new approach provides a rapid and sensitive method for the detection of chloroquine using hydrophilic interaction LC coupled to MS/MS (HILIC–MS/MS). Ammonium formate and formic acid were added to mobile phase to attain good peak shapes and the salified chloroquine as well retained in an HILIC column. Linearity, intra- and inter-day precision, accuracy, recovery, matrix effect and stability were evaluated during the validation process.Conclusion: The validated method has been successfully used in a PK study in miniature pigs, and paves way for future development.