MicroRNA-210 regulates placental adaptation to maternal hypoxic stress during pregnancy. Issue 2 (19th October 2020)

• Record Type: Journal Article
• Authors: Bian, Xiaotao; Liu, Juan; Yang, Qian; Liu, Yanlei; Jia, Wentong; Zhang, Xiaodong; Li, Yu-Xia; Shao, Xuan; Wang, Yan-Ling
• Is Part Of: Biology of reproduction. Volume 104:Issue 2(2021)
• Page Start: 418
• Page End: 429
• Publication Date: 2020-10-19
• Subjects: miR-210 -- knockout mice -- maternal hypoxia -- embryo growth -- placental adaptation; Reproduction -- Periodicals; Biology; Reproduction; Reproduction; Electronic journals; Periodicals; Periodicals; 571.805
• DOI: 10.1093/biolre/ioaa187
• Languages: English
• ISSNs: 0006-3363

Abstract

Abstract: MicroRNA (miR)-210 is a well-known hypoxia-inducible small RNA. Increasing in vitro evidence demonstrates its involvement in regulating multiple behaviors of placental trophoblasts. However, direct in vivo evidence remains lacking. In the present study, we generated a miR-210-deficient mouse strain using CRISPR/Cas9 technology, in which miR-210 expression was markedly deficient in various tissues. Little influence on fertility rate and litter size was observed after the deletion of miR-210 in mice. Continuous exposure of pregnant mice to hypoxia (10.5% O2 ) from E6.5 to E10.5 or to E18.5 led to reduction in fetal weight, and such fetal weight loss was markedly worsened in miR-210-knockout dams. Analysis of the placental structure demonstrated the reduced expansion of placental spongiotrophoblast layer and hampered development of labyrinth fetal blood vessels in knockout mice compared to the wild-type controls upon hypoxia stimulation. The findings indicate that miR-210 participates in regulating placental adaptation to hypoxic stress during pregnancy. Abstract : Summary Sentence MicroRNA-210 is involved in modulating placental adaptation to maternal hypoxia to support fetal growth under unfavorable environment.