A5.16 Characterisation of an arthritis rat model with a periodontal disease induced by Porphyromonas Gingivalis. (13th February 2015)

• Record Type: Journal Article
• Authors: Rinaudo-Gaujous, M; Courbon, G; Blasco-Baque, V; Paul, S; Marotte, H
• Is Part Of: Annals of the rheumatic diseases. Volume 74(2015)Supplement 1
• Page Start: A53
• Page End: A53
• Publication Date: 2015-02-13
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2015-207259.122
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background and objectives: Epidemiological studies have demonstrated the association between rheumatoid arthritis (RA) and periodontal disease (PD) susceptibility and severity. The missing link between RA and PD association could be Porphyromonas gingivalis ( PG ) involved in PD pathogenesis. Role of PG is growing fast since it is the only bacteria able to citrullinate peptides and could induce autoimmune response through development of anti-citrullinated protein antibodies (ACPA). We have already demonstrated that anti- PG antibodies were correlated with ACPA in RA patients. The aim of this study is to develop a physiopathological rat model of PD induced by PG infection to follow the occurrence of early signs of RA and to score bone loss. Materials and methods: Female rats Lewis were randomly divided into two groups: oral infection with PG or negative control. The oral infection was performed by oral gavage with PG during 1 month. Clinical parameters including articular index were monitored during the experiment. Blood samples were collected at different time point for determination of ACPA, RF, MMP-3 (biomarker for RA and PD destruction) and anti- PG antibodies by ELISA assay. Count of activated T lymphocytes that secrete IL-17 was performed by ELISPOT. Bone microarchitecture, bone mineral density (BMD) profiles were obtained by µ-computed tomography (µ-CT) and dual energy x-rays absorptiometry (DXA) x-rays, respectively. Results: ELISA assay for ACPA determination in rats is set up and correlation between arthritis and ACPA has been established (p < 0.01). Moreover, ACPA is found in early phase of arthritis compared to controls (p < 0.01). Development of RF, MMP-3 assay, ELISPOT and radiological evaluation are still ongoing. Conclusions: The development of a reliable animal model of arthritis induced by periodontal disease with PG is essential for the test of new therapeutics in the future.