Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction. (9th November 2018)

• Record Type: Journal Article
• Authors: Cha, Hyun-Hwa; Hwang, Jae-Ryoung; Sung, Ji Hee; Choi, Suk-Joo; Oh, Soo-young; Roh, Cheong-Rae
• Is Part Of: Journal of investigative medicine. Volume 67:Number 2(2019)
• Page Start: 319
• Page End: 326
• Publication Date: 2018-11-09
• Subjects: calcium -- plasma membrane Ca2+-ATPase (PMCA) -- sarcoendoplasmic reticulum CA2+-ATPase -- pre-eclampsia -- placentas; Clinical medicine -- Periodicals; Medicine -- Research -- Periodicals; Medicine; Research -- United States; Clinical medicine; Medicine -- Research; Periodicals; 616.075
• DOI: 10.1136/jim-2018-000844
• Languages: English
• ISSNs: 1081-5589

Abstract

Abstract : We aimed to evaluate the changes in plasma membrane Ca 2+ -ATPase (PMCA) and sarcoendoplasmic reticulum CA 2+ -ATPase (SERCA-2) according to the antepartal magnesium sulfate (MgSO4 ) administration in the placentas from pregnancies with pre-eclampsia (PE) or fetal growth restriction (FGR). Pregnant women were classified as follows: (group 1) pregnancies without PE or FGR (n=16), (group 2) pregnancies with PE or FGR but without MgSO4 administration (n=14), and (group 3) pregnancies with PE or FGR and with MgSO4 administration (n=28). We observed the localization of PMCA and SERCA-2 in placentas and compared its expression among 3 groups. And we observed its expression in BeWo cells following treatment with MgSO4 and CoCl2 . PMCA staining was more observed in the basal membrane, whereas SERCA-2 staining was observed predominantly under the microvillous membrane. SERCA-2 expression was significantly increased in group 3 compared with that in group 1. Considering the gestational age at delivery, PMCA expression was increased in group 2 and group 3 compared with that in group 1 after 36 weeks of gestation. SERCA-2 was increased in group 3, but not in group 2 compared with that in group 1 after 36 weeks of gestation. In BeWo cells, MgSO4 treatment increased PMCA and SERCA-2 expression. PMCA expression was influenced by gestational age at delivery, and SERCA-2 expression was increased in the presence of PE and antepartal MgSO4 administration. This indicates that antepartal MgSO4 administration has a greater influence on SERCA-2 than PMCA.