Supplementation of CD4+CD25+ regulatory T cells suppresses experimental autoimmune uveoretinitis. Issue 1 (30th August 2006)

• Record Type: Journal Article
• Authors: Keino, H; Takeuchi, M; Usui, Y; Hattori, T; Yamakawa, N; Kezuka, T; Sakai, J-I; Usui, M
• Is Part Of: British journal of ophthalmology. Volume 91:Issue 1(2007)
• Page Start: 105
• Page End: 110
• Publication Date: 2006-08-30
• Subjects: CFA, complete Freund's adjuvant -- EAU, experimental autoimmune uveoretinitis -- IFN, interferon -- IRBP, interphotoreceptor retinoid-binding protein -- IRBP1–20, interphotoreceptor retinoid-binding protein peptide 1–20 -- PCR, polymerase chain reaction -- TCR, T cell receptor -- TGF, transforming growth factor; Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.2006.099192
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Aims: To investigate whether supplementation of natural CD4+CD25+ regulatory T cells ameliorates mouse experimental autoimmune uveoretinitis (EAU) induced by CD4+ T cell-dependent interphotoreceptor retinoid-binding protein (IRBP). Methods: C57BL/6 mice were immunised with human interphotoreceptor retinoid-binding protein peptide 1–20 (IRBP1–20 ), and IRBP1–20 -sensitised T cells were obtained. CD4+CD25+ T cells derived from naive mice were cocultured with IRBP1–20 -sensitised T cells, and their proliferation responses and cytokine production were measured. In addition, CD4+CD25+ T cells were transferred intravenously into mice 7 or 15 days after immunisation with IRBP1–20, and the severity of EAU and T cell proliferation responses were evaluated. Results: CD4+CD25+ regulatory T cells effectively inhibited both the proliferation of, and interleukin (IL)2, IL5 and interferon (IFN)γ production by, IRBP1–20 -sensitised T cells. Adoptive transfer of CD4+CD25+ regulatory T cells to IRBP1–20 -immunised mice conferred considerable protection from EAU development and inhibition of T cell proliferation responses to IRBP1–20 . Conclusion: These findings show that natural CD4+CD25+ regulatory T cells possess the ability to inhibit activation of IRBP-reactive T cells that have been already sensitised in vivo, and adoptive transfer of these cells ameliorates EAU even in the effector phase. Supplementation of natural CD4+CD25+ regulatory T cells may have therapeutic potential for effective treatment of uveitis.