Differential responses of the green microalga Chlorella zofingiensis to the starvation of various nutrients for oil and astaxanthin production. (February 2018)

• Record Type: Journal Article
• Authors: Mao, Xuemei; Wu, Tao; Sun, Dongzhe; Zhang, Zhao; Chen, Feng
• Is Part Of: Bioresource technology. Volume 249(2018)
• Page Start: 791
• Page End: 798
• Publication Date: 2018-02
• Subjects: Chlorella zofingiensis -- Lipid -- Astaxanthin -- Nutrient starvation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.10.090
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: NS and SS could trigger the accumulation of TAG and astaxanthin in C. zofingiensis . Highest TAG and astaxanthin productivity were reached under SS and NS respectively. A working model for stress-associated TAG and astaxanthin synthesis was proposed. Abstract: Chlorella zofingiensis has been proposed as a potential producer of lipids and the high-value carotenoid astaxanthin. In this study, the responses of photoautotrophic C. zofingiensis with respect to growth, lipid profiles and astaxanthin accumulation were investigated upon the starvation of N (NS), P (PS) and S (SS). NS and SS stimulated triacylglycerol (TAG) accumulation, which reached 27% and 21% of dry weight (DW), respectively. Stresses also stimulated astaxanthin accumulation greatly, reaching 3.9 mg/g DW by NS. SS led to the highest TAG productivity (52.4 mg L −1 d −1 ) while NS gave rise to the highest astaxanthin productivity (0.624 mg L −1 d −1 ). In combination with transcriptional analysis, a working model for stress-associated TAG and astaxanthin biosynthesis was proposed. Taken together, these detailed data shed light on the elucidation of differential responses to nutrient stresses and may provide insights into future engineering of this promising alga for improving TAG and astaxanthin production.