Comparative analysis of transcriptomes from different coloration of Chinese mitten crab Eriocheir sinensis. Issue 98 (March 2020)

• Record Type: Journal Article
• Authors: Zhao, Yingying; Zhu, Xiaochen; Han, Zhibin; Zhang, Yazhao; Dong, Tengfei; Li, Yingdong; Dong, Jing; Wei, Hua; Li, Xiaodong
• Is Part Of: Fish & shellfish immunology. Issue 98(2020)
• Page Start: 515
• Page End: 521
• Publication Date: 2020-03
• Subjects: Eriocheir sinensis -- Comparative transcriptome -- Different expressed genes -- Immune-related gene -- Color-related gene; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.01.051
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Chinese mitten crab Eriocheir sinensis is probably the most important freshwater cultured crab in China. A tiny minority of brownish-orange individuals have been discovered in the long period of artificial breeding history of E. sinensis s. Those mutants are usually accompanied with slow growth rate, low molting frequency and poor survival rate, which may be the results of growth defects and immunodeficiency. To better understand the relationship between body color determination and the immune system as well as the related genes expression in E. sinensis s, we performed the whole-body transcriptome analysis in different color of first stage zoea (ZI) larvae using next-generation sequencing (NGS) technology. We randomly assembled 175.40 and 177.52million clean reads from the wild and mutant ZIs, respectively. Finally, we identified 7153 differentially expressed genes (DEGs) ( p < 0.05), with 5194 up-regulated and 1959 down-regulated. A total of 13 KEGG pathways related to immune system were detected among 248 pathways. Except the first whole-body RNA sequencing of color-specific transcriptomes for E. sinensis, this study will offer a better understanding of the underlying molecular mechanisms of interaction between color determination and the immune system. Highlights: First whole-body RNA sequencing of color-specific transcriptomes for Eriocheir sinensis were studied. Some new candidate genes between different color types of E. sinensis were spot. Some immunity related DEGs were determined and analyzed, and confirmed by qRT-PCR.