Pharmacological safety of Plinia cauliflora (Mart.) Kausel in rabbits. (2019)

• Record Type: Journal Article
• Authors: Palozi, Rhanany Alan Calloi; Guarnier, Lucas Pires; Romão, Paulo Vitor Moreira; Nocchi, Samara Requena; dos Santos, Carlos Calixto; Lourenço, Emerson Luiz Botelho; Silva, Denise Brentan; Gasparotto, Francielly Mourão; Gasparotto Junior, Arquimedes
• Is Part Of: Toxicology reports. Volume 6(2019)
• Page Start: 616
• Page End: 624
• Publication Date: 2019
• Subjects: ABG Arterial blood gas -- ANOVA One-way analysis of variance -- ASE Accelerated solvent extraction -- BB Buffer Base -- BE Base Excess -- BEecf Base excess in the extracellular fluid compartment -- Ca++ Calcium -- cHCO3 Bicarbonate concentration -- Cl Chloride -- CNS Central nervous system -- ctCO2 (B) Concentration of total carbon dioxide of whole blood -- ctCO2 (P) Concentration of total carbon dioxide in plasma -- ctO2 Concentration of total oxygen -- DBP Diastolic blood pressure -- ECG Electrocardiography -- EEPC Ethanol extract of Plinia cauliflora -- GAE Gallic acid equivalent -- H+ Hydrogen ion dissociated -- Hct Hematocrit -- HHb Deoxyhemoglobin -- K+ Potassium -- LA Left arm -- LC-DAD-MS Liquid chromatography coupled to a diode array detector and mass spectrometer -- LL Left leg -- MAP Mean arterial pressure -- Na+ Sodium -- Na₂CO₃ Sodium carbonate -- O2Hb Oxyhemoglobin -- P50 Half of the maximum hemoglobin saturation -- PCO2 Partial pressure of carbon dioxide -- pH Potential of hydrogen -- PO2 Partial pressure of oxygen -- RA Right arm -- RL Right leg -- S.E.M Standard error of the mean -- SBP Systolic blood pressure -- SO2 Level of hemoglobin-saturation by oxygen -- tHb Hemoglobin -- UFLC Ultra fast liquid chromatograph; Cardiovascular -- Irwin test -- Myrtaceae -- Respiratory -- Toxicology; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2019.06.017
• Languages: English
• ISSNs: 2214-7500

Abstract

Graphical abstract: Highlights: Fruit peels of Plinia cauliflora are widely used in Brazilian traditional medicine. No studies have proved the safety of its pharmacological effects. We assessed the safety pharmacology of P. cauliflora extract (EEPC) in rabbits. EEPC did not cause any significant changes in several physiological systems. These data provide important safety data for its clinical use. Abstract: Fruit peels of Plinia cauliflora (Mart.) Kausel are widely used in Brazilian traditional medicine, but no studies have proved the safety of its pharmacological effects on the respiratory, cardiovascular, and central nervous systems. The present study assessed the safety pharmacology of P. cauliflora in New Zealand rabbits. First, an ethanol extract (EEPC) was selected for the pharmacological experiments and chemical characterization. Then, different groups of rabbits were orally treated with EEPC (200 and 2000 mg/kg) or vehicle. Acute behavioral and physiological alterations in the modified Irwin test, respiratory rate, arterial blood gas, and various cardiovascular parameters (i.e., heart rate, blood pressure, and electrocardiography) were evaluated. The main secondary metabolites that were identified in EEPC were ellagic acid, gallic acid, O -deoxyhexosyl quercetin, and the anthocyanin O -hexosyl cyanidin. No significant behavioral or physiological changes were observed in any of the groups. None of the doses of EEPC affected respiratory rate or arterial blood gas, with no changes on blood pressure or electrocardiographic parameters. The present study showed that EEPC did not cause any significant changes in respiratory, cardiovascular, or central nervous system function. These data provide scientific evidence of the effects of this species and important safety data for its clinical use.