Zinc alleviates arsenism in common carp: Varied change profiles of cytokines and tight junction proteins among two intestinal segments. Issue 94 (November 2019)

• Record Type: Journal Article
• Authors: Zhao, Hongjing; Wang, Yu; Yang, Xin; Fei, Dongxue; Mu, Mengyao; Guo, Menghao; Yu, Hongxian; Xing, Mingwei
• Is Part Of: Fish & shellfish immunology. Issue 94(2019)
• Page Start: 761
• Page End: 768
• Publication Date: 2019-11
• Subjects: Intestinal regions -- Arsenic -- Zinc -- Inflammation -- Tight junction; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.09.069
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: This study was designed to evaluate the effects of zinc on inflammation and tight junction (TJ) in different intestinal regions of common carp under sub-chronic arsenic insult. Fish were exposed to zinc (0, 1 mg/L) and arsenic trioxide (0, 2.83 mg/L) in individual or combination for a month. Inflammatory infiltration and TJ structure changes were displayed by H&E staining and transmission electron microscope. To further explore these changes, biochemical indicator (SOD), gene or protein expressions of inflammatory responses (NF-κB, IL-1β, IL-6 and IL-8) and TJ proteins (Occludin, Claudins and ZOs) were determined. In the anterior intestine, arsenic decreased activity of SOD, mRNA levels of Occludin, Claudins and ZOs, increased mRNA levels of ILs. However, unlike the anterior intestine, arsenic has an upregulation effects of Occludin and Claudin-4 in the mid intestine. These anomalies induced by arsenic, except IL-8, were completely or partially recovered by zinc co-administration. Furthermore, transcription factor (NF-κB) nuclear translocation paralleled with its downstream genes in both intestinal regions. In conclusion, our results unambiguously suggested that under arsenic stress, zinc can partly relieve intestinal inflammation and disruption of tight junction segment-dependently. Graphical abstract: Image 1 Highlights: Arsenic induces inflammation and tight junction proteins breakdown in the intestines of common fish. Zinc alleviates arsenic-induced adverse effects via NF-κB pathway. Mid intestine has a strong ability to resist to arsenic stress. Zinc exerts superior prophylactic effect in anterior intestinal region.