Identification and characterization of a novel L-type lectin (MjLTL2) from kuruma shrimp (Marsupenaeus japonicus). Issue 98 (March 2020)

• Record Type: Journal Article
• Authors: Xu, Sen; Jing, Ming; Liu, Wen-Ying; Dong, He; Kong, De-Min; Wang, Ya-Ru; Zhang, Han-Han; Yue, Zhen; Li, You-Jie; Jiao, Fei; Xie, Shu-Yang
• Is Part Of: Fish & shellfish immunology. Issue 98(2020)
• Page Start: 354
• Page End: 363
• Publication Date: 2020-03
• Subjects: Innate immunity -- L-type lectin -- Marsupenaeus japonicus -- WSSV -- Vibrio anguillarum; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.01.022
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: L-type lectins (LTLs) belong to the lectin family and are characterized by a conserved structural motif in their carbohydrate recognition domain. LTLs are homologous to leguminous lectins. In this study, we identified and functionally characterized an LTL from kuruma shrimp Marsupenaeus japonicus . We designated this LTL as Mj LTL2. Mj LTL2 contains a signal peptide, a Lectin_leg domain, a coiled coil, and transmembrane domain. Mj LTL2 is distributed in hemocytes, heart, hepatopancreas, gill, stomach, and intestine; higher expression levels are seen in hemocytes and the hepatopancreas than in other tissues. Mj LTL2 was upregulated following challenge of shrimp with Vibrio anguillarum and white spot syndrome virus (WSSV). Mj LTL2 can agglutinate several bacteria without Ca 2+ . In addition, Mj LTL2 could bind to several Gram-positive and -negative bacteria by binding to their lipopolysaccharide and peptidoglycan. However, Mj LTL2 could not enhance the clearance of V. anguillarum in vivo. In the presence of WSSV infection, Mj LTL2 knockdown by RNA interference resulted in a 7-day lower cumulative mortality of M. japonicus . Moreover, less VP19, VP24, VP26, and VP2 8 mRNAs were extracted from the hemocytes of Mj LTL2 knockdown shrimp than from the control. These results suggest that Mj LTL2 is involved in immune responses in shrimp. Highlights: Mj LTL2 could agglutinate several Gram-positive and -negative bacteria. Mj LTL2 could bind to several Gram-positive and -negative bacteria. Mj LTL2 could bind to lipopolysaccharide and peptidoglycan. MjLTL2 knockdown resulted in a lower cumulative mortality of M. japonicus in the presence of WSSV infection. MjLTL2 knockdown resulted in a lower VP19, VP24, VP26, and VP28 expression levels.